HCV NS5a Protein (AA 2210-2306) (His tag)
HCV NS5a
Origin: Hepatitis C Virus (HCV)
Host: Escherichia coli (E. coli)
Recombinant
> 90 % by SDS - PAGE
SDS
1 image
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- Target See all HCV NS5a products
- HCV NS5a (Hepatitis C Virus NS5a (HCV NS5a))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 2210-2306
- Origin
- Hepatitis C Virus (HCV)
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This HCV NS5a protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Sequence
- MGSSHHHHHH SSGLVPRGSH MSLKATCTAN HDSPDAELIE ANLLWRQEMG GNITRVESEN KVVILDSFDP LVAEEDEREV SVPAEILRKS RRFARALPVW ARPDYNPPLV ETWKKPDY
- Purity
- > 90 % by SDS - PAGE
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.25 mg/mL
- Buffer
- Liquid. In 20 mM Tris-HCl buffer ( pH 8.0) containing 0.2M NaCl, 40 % glycerol, 1 mM DTT
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- Target
- HCV NS5a (Hepatitis C Virus NS5a (HCV NS5a))
- Alternative Name
- HCV-NS5a (HCV NS5a Products)
- Target Type
- Viral Protein
- Background
- NS5A is a component of the replication complex involved in RNA-binding. Its interaction with Human VAPB may target the viral replication complex to vesicles. This protein down-regulates viral IRES translation initiation and mediates interferon resistance, presumably by interacting with and inhibiting human EIF2AK2/PKR. NS5a seems to inhibit apoptosis by interacting with BIN1 and FKBP8. The hyperphosphorylated form of NS5A is an inhibitor of viral replication. Recombinant HCV(Hepatitis C Virus) NS5A protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
- Molecular Weight
- 13.4 kDa (118aa) confirmed by MALDI-TOF
- NCBI Accession
- NP_671491
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