EEF2K Protein (AA 1-725) (His tag)
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- Target See all EEF2K Proteins
- EEF2K (Eukaryotic Elongation Factor-2 Kinase (EEF2K))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-725
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This EEF2K protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Sequence
- MGSSHHHHHH SSGLVPRGSH MGSMADEDLI FRLEGVDGGQ SPRAGHDGDS DGDSDDEEGY FICPITDDPS SNQNVNSKVN KYYSNLTKSE RYSSSGSPAN SFHFKEAWKH AIQKAKHMPD PWAEFHLEDI ATERATRHRY NAVTGEWLDD EVLIKMASQP FGRGAMRECF RTKKLSNFLH AQQWKGASNY VAKRYIEPVD RDVYFEDVRL QMEAKLWGEE YNRHKPPKQV DIMQMCIIEL KDRPGKPLFH LEHYIEGKYI KYNSNSGFVR DDNIRLTPQA FSHFTFERSG HQLIVVDIQG VGDLYTDPQI HTETGTDFGD GNLGVRGMAL FFYSHACNRI CESMGLAPFD LSPRERDAVN QNTKLLQSAK TILRGTEEKC GSPRVRTLSG SRPPLLRPLS ENSGDENMSD VTFDSLPSSP SSATPHSQKL DHLHWPVFSD LDNMASRDHD HLDNHRESEN SGDSGYPSEK RGELDDPEPR EHGHSYSNRK YESDEDSLGS SGRVCVEKWN LLNSSRLHLP RASAVALEVQ RLNALDLEKK IGKSILGKVH LAMVRYHEGG RFCEKGEEWD QESAVFHLEH AANLGELEAI VGLGLMYSQL PHHILADVSL KETEENKTKG FDYLLKAAEA GDRQSMILVA RAFDSGQNLS PDRCQDWLEA LHWYNTALEM TDCDEGGEYD GMQDEPRYMM LAREAEMLFT GGYGLEKDPQ RSGDLYTQAA EAAMEAMKGR LANQYYQKAE EAWAQMEE
- Purity
- > 85 % by SDS - PAGE
- Top Product
- Discover our top product EEF2K Protein
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Eukaryotic Elongation Factor-2 Kinase (EEF2K) (AA 1-725) protein (GST tag)
EEF2K Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP, KAA
Eukaryotic Elongation Factor-2 Kinase (EEF2K) (AA 1-725) (Active) protein (GST tag)EEF2K Origin: Human Host: Escherichia coli (E. coli) Recombinant SDS, Func Active
Eukaryotic Elongation Factor-2 Kinase (EEF2K) (AA 2-725) protein (His tag)Crystallography grade EEF2K Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Eukaryotic Elongation Factor-2 Kinase (EEF2K) (AA 2-724) protein (His tag)Crystallography grade EEF2K Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Eukaryotic Elongation Factor-2 Kinase (EEF2K) protein (Myc-DYKDDDDK Tag)EEF2K Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.25 mg/mL
- Buffer
- Liquid. In Phosphate buffered saline ( pH 7.4), 10 % glycerol
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- Target
- EEF2K (Eukaryotic Elongation Factor-2 Kinase (EEF2K))
- Alternative Name
- EEF2K (EEF2K Products)
- Synonyms
- HSU93850 Protein, eEF-2K Protein, C86191 Protein, SMEF2K Protein, eEF-2 kinase Protein, cb365 Protein, fa04b08 Protein, wu:fa04b08 Protein, zgc:100973 Protein, eukaryotic elongation factor 2 kinase Protein, eukaryotic elongation factor-2 kinase Protein, EEF2K Protein, Eef2k Protein, eef2k Protein, LOC101930123 Protein
- Background
- Eukaryotic elongation factor-2 kinase, also known as EEF2K, is threonine kinase that regulates protein synthesis by controlling the rate of peptide chain elongation. upon activation by a variety of upstream kinases including AMPK or TRPM7, phosphorylates the elongation factor EEF2 at a single site, renders it unable to bind ribosomes and thus inactive. Recombinant human EEF2K protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
- Molecular Weight
- 84.6 kDa (748aa)
- NCBI Accession
- NP_037434
- UniProt
- O00418
- Pathways
- AMPK Signaling
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