NARS Protein (AA 1-548) (His tag)

Catalog No. ABIN6387628

Product Details

Target: NARS (Asparaginyl-tRNA Synthetase (NARS))

Protein Type: Recombinant

Protein Characteristics: AA 1-548

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This NARS protein is labelled with His tag.

Application: SDS-PAGE (SDS)

Sequence: MGSSHHHHHH SSGLVPRGSH MGSMVLAELY VSDREGSDAT GDGTKEKPFK TGLKALMTVG KEPFPTIYVD SQKENERWNV ISKSQLKNIK KMWHREQMKS ESREKKEAED SLRREKNLEE AKKITIKNDP SLPEPKCVKI GALEGYRGQR VKVFGWVHRL RRQGKNLMFL VLRDGTGYLQ CVLADELCQC YNGVLLSTES SVAVYGMLNL TPKGKQAPGG HELSCDFWEL IGLAPAGGAD NLINEESDVD VQLNNRHMMI RGENMSKILK ARSMVTRCFR DHFFDRGYYE VTPPTLVQTQ VEGGATLFKL DYFGEEAFLT QSSQLYLETC LPALGDVFCI AQSYRAEQSR TRRHLAEYTH VEAECPFLTF DDLLNRLEDL VCDVVDRILK SPAGSIVHEL NPNFQPPKRP FKRMNYSDAI VWLKEHDVKK EDGTFYEFGE DIPEAPERLM TDTINEPILL CRFPVEIKSF YMQRCPEDSR LTESVDVLMP NVGEIVGGSM RIFDSEEILA GYKREGIDPT PYYWYTDQRK YGTCPHGGYG LGLERFLTWI LNRYHIRDVC LYPRFVQRCT P

Purity: > 80 % by SDS - PAGE

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: Liquid. In Phosphate Buffered Saline (  pH 7.4) containing 10 % glycerol

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

Target Details

Target: NARS (Asparaginyl-tRNA Synthetase (NARS))

Alternative Name: NARS (NARS Products)

Synonyms: ASNRS Protein, NARS1 Protein, AsnRS Protein, BcDNA:GH06451 Protein, CG10687 Protein, Ca Protein, Dmel\\CG10687 Protein, NRS Protein, l(2)37Ca Protein, l(2)37Ca)E11 Protein, l(2)E11 Protein, 3010001M15Rik Protein, AA960128 Protein, C78150 Protein, LRRGT00113 Protein, fb57a10 Protein, wu:fb57a10 Protein, si:dkey-274m14.2 Protein, BA4802 Protein, asparaginyl-tRNA synthetase Protein, Asparaginyl-tRNA synthetase Protein, asparagine--tRNA ligase Protein, asparaginyl-tRNA synthetase AsnS Protein, asparaginyl-tRNA ligase Protein, asparaginyl-tRNA synthetase 2, mitochondrial Protein, asparagine--tRNA ligase DED81 Protein, asparaginyL-tRNA synthetase Protein, NARS Protein, AsnRS Protein, Nars Protein, nars Protein, asnS Protein, asnC Protein, GYMC10_2250 Protein, Mesil_2042 Protein, Trad_1774 Protein, NARS2 Protein, DED81 Protein, Ilyop_2011 Protein, Palpr_1926 Protein, Ocepr_1116 Protein, Olsu_0392 Protein

Background: NARS also known as asparagine--tRNA ligase, cytoplasmic. This protein acts as an enzyme. It is known to catalyze the following reaction: ATP + L-asparagine + tRNA(Asn) = AMP + diphosphate + L-asparaginyl-tRNA(Asn). Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. Asparaginyl-tRNA synthetase is localized to the cytoplasm and belongs to the class II family of tRNA synthetases. The N-terminal domain represents the signature sequence for the eukaryotic asparaginyl-tRNA synthetases. Recombinant human NARS, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques

Molecular Weight: 65.3kDa (571aa)

NCBI Accession: NP_004530

UniProt: O43776