CSTB Protein (AA 1-98) (His tag)
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- Target See all CSTB Proteins
- CSTB (Cystatin B (Stefin B) (CSTB))
- Protein Type
- Recombinant
- Biological Activity
- Active
- Protein Characteristics
- AA 1-98
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This CSTB protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Sequence
- MGSSHHHHHH SSGLVPRGSH MMCGAPSATQ PATAETQHIA DQVRSQLEEK ENKKFPVFKA VSFKSQVVAG TNYFIKVHVG DEDFVHLRVF QSLPHENKPL TLSNYQTNKA KHDELTYF
- Purity
- > 95 % by SDS - PAGE
- Endotoxin Level
- < 1.0 EU per 1 microgram of protein (determined by LAL method)
- Biological Activity Comment
- The IC50 value is < 3.0nM. The inhibitory function of Cystatin B on protease activity of papain was measured by a fluorometric assay using Z-FR-AMC at pH 7.5 at 25C.
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Cystatin B (Stefin B) (CSTB) (AA 1-98) protein (GST tag)
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Cystatin B (Stefin B) (CSTB) (AA 1-98), (full length) protein (GST tag)CSTB Origin: Human Host: Escherichia coli (E. coli) Recombinant 95 % SDS, WB, ELISA
Cystatin B (Stefin B) (CSTB) proteinCSTB Origin: Cow Host: Escherichia coli (E. coli) Recombinant > 95 % SDS, WB, ELISA, IP
Cystatin B (Stefin B) (CSTB) protein (Myc-DYKDDDDK Tag)CSTB Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
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Bioactivity Validated
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- Liquid. In 20 mM Tris-HCl buffer ( pH 8.0) containing 50 mM NaCl
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- Target
- CSTB (Cystatin B (Stefin B) (CSTB))
- Alternative Name
- CSTB (CSTB Products)
- Synonyms
- CST6 Protein, EPM1 Protein, EPM1A Protein, PME Protein, STFB Protein, ULD Protein, CSTB Protein, Cyb Protein, Epm1 Protein, Stfb Protein, MGC189005 Protein, pme Protein, cst6 Protein, epm1 Protein, stfb Protein, AA960480 Protein, ARABIDOPSIS THALIANA PHYTOCYSTATIN 6 Protein, ATCYS6 Protein, ATCYSB Protein, cystatin B Protein, LOC100008600 Protein, LOC100136235 Protein, CPI1 Protein, cystatin B Protein, cystatin B (stefin B) Protein, cystatin 14a, tandem duplicate 2 Protein, cystatin-B Protein, CSTB Protein, Cstb Protein, cstb Protein, cst14a.2 Protein, CYSB Protein, cpi1 Protein, LOC101123265 Protein
- Background
- CSTB, also known as Cystatin B is an anti-protease implicated in myoclonus epilepsy, a degenerative disease of the central nervous system. The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. This protein is able to form a dimer stabilized by noncovalent forces and is thought to play a role in protecting against the proteases leaking from lysosomes. In cells, CSTB is located in the lysosomes and the cytoplasm, but also in the nucleus. Recombinant CSTB, fused to His-tag, was expressed in E.coli and purified by using conventional chromatography techniques.
- Molecular Weight
- 13 kDa (118aa) confirmed by MALDI-TOF
- NCBI Accession
- NP_000091
- UniProt
- P04080
- Pathways
- Response to Water Deprivation
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