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CSTB Protein (His tag)

CSTB Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % as determined by reducing SDS-PAGE. Active
Catalog No. ABIN7317867
  • Target See all CSTB Proteins
    CSTB (Cystatin B (Stefin B) (CSTB))
    Protein Type
    Recombinant
    Biological Activity
    Active
    Origin
    • 5
    • 3
    • 3
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Source
    • 9
    • 7
    • 1
    • 1
    • 1
    Escherichia coli (E. coli)
    Purification tag / Conjugate
    This CSTB protein is labelled with His tag.
    Purpose
    Recombinant Human Cystatin B/CSTB Protein (His Tag)(Active)
    Sequence
    Met 2-Phe 98
    Characteristics
    A DNA sequence encoding the human CSTB (P04080) (Met 2-Phe 98) was expressed, with a polyhistide tag at the N-terminus.
    Purity
    > 95 % as determined by reducing SDS-PAGE.
    Biological Activity Comment
    Measured by its ability to inhibit papain cleavage of a fluorogenic peptide substrate Z-FR-AMC (R&D Systems, Catalog # ES009). The IC50 value is < 15 nM.
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    Discover our top product CSTB Protein
  • Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    Please refer to the printed manual for detailed information.
    Buffer
    Lyophilized from sterile 50 mM Tris, 50 mM NaCl, pH 8.0
    Storage
    4 °C,-20 °C,-80 °C
    Storage Comment
    Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
  • Target
    CSTB (Cystatin B (Stefin B) (CSTB))
    Alternative Name
    Cystatin B/CSTB (CSTB Products)
    Synonyms
    CST6 Protein, EPM1 Protein, EPM1A Protein, PME Protein, STFB Protein, ULD Protein, CSTB Protein, Cyb Protein, Epm1 Protein, Stfb Protein, MGC189005 Protein, pme Protein, cst6 Protein, epm1 Protein, stfb Protein, AA960480 Protein, ARABIDOPSIS THALIANA PHYTOCYSTATIN 6 Protein, ATCYS6 Protein, ATCYSB Protein, cystatin B Protein, LOC100008600 Protein, LOC100136235 Protein, CPI1 Protein, cystatin B Protein, cystatin B (stefin B) Protein, cystatin 14a, tandem duplicate 2 Protein, cystatin-B Protein, CSTB Protein, Cstb Protein, cstb Protein, cst14a.2 Protein, CYSB Protein, cpi1 Protein, LOC101123265 Protein
    Background

    Background: Cystatin-B, also known as CPI-B, Liver thiol proteinase inhibitor, Stefin-B, CSTB and CST6, is a cytoplasm and nucleus protein which belongs to the cystatin family. Cystatin-B / CSTB is an intracellular thiol proteinase inhibitor. Tightly binding reversible inhibitor of cathepsins L, H and B. Cystatin-B / CSTB is able to form a dimer stabilized by noncovalent forces, inhibiting papain and cathepsins l, h and b. Cystatin-B / CSTB is also thought to play a role in protecting against the proteases leaking from lysosomes. Defects in Cystatin-B / CSTB are the cause of progressive myoclonic epilepsy type 1 (EPM1) which is an autosomal recessive disorder characterized by severe, stimulus-sensitive myoclonus and tonic-clonic seizures. The cystatins are a family of cysteine protease inhibitors with homology to chicken cystatin. Cystatins are physiological inhibitors of cysteine proteinases which are widely distributed in human tissues and fluids. Cystatins typically comprise about 115 amino acids, are largely acidic, contain four conserved cysteine residues known to form two disulfide bonds. Cystatins may be glycosylated and / or phosphorylated, with similarity to fetuins, kininogens, stefins, histidine-rich glycoproteins and cystatin-related proteins. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired inhibitory activity. Cystatins mainly inhibit peptidases belonging to peptidase families C1 (papain family) and C13 (legumain family).

    Synonym: CST6;EPM1;EPM1A;PME;STFB;ULD

    Molecular Weight
    12.5 kDa
    UniProt
    P04080
    Pathways
    Response to Water Deprivation
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