PGK2 Protein (AA 1-417) (His tag)
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- Target See all PGK2 Proteins
- PGK2 (Phosphoglycerate Kinase 2 (PGK2))
- Protein Type
- Recombinant
- Biological Activity
- Active
- Protein Characteristics
- AA 1-417
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This PGK2 protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Sequence
- MGSSHHHHHH SSGLVPRGSH MSLSKKLTLD KLDVRGKRVI MRVDFNVPMK KNQITNNQRI KASIPSIKYC LDNGAKAVVL MSHLGRPDGV PMPDKYSLAP VAVELKSLLG KDVLFLKDCV GAEVEKACAN PAPGSVILLE NLRFHVEEEG KGQDPSGKKI KAEPDKIEAF RASLSKLGDV YVNDAFGTAH RAHSSMVGVN LPHKASGFLM KKELDYFAKA LENPVRPFLA ILGGAKVADK IQLIKNMLDK VNEMIIGGGM AYTFLKVLNN MEIGASLFDE EGAKIVKDIM AKAQKNGVRI TFPVDFVTGD KFDENAQVGK ATVASGISPG WMGLDCGPES NKNHAQVVAQ ARLIVWNGPL GVFEWDAFAK GTKALMDEIV KATSKGCITV IGGGDTATCC AKWNTEDKVS HVSTGGGASL ELLEGKILPG VEALSNM
- Purity
- > 95 % by SDS - PAGE
- Biological Activity Comment
- Specific activity is > 500 units/mg. One unit will convert 1 umole of 1,3-Bisphosphoglycerate to 3-PGA per minute at pH 8.0 at 37C.
- Top Product
- Discover our top product PGK2 Protein
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Phosphoglycerate Kinase 2 (PGK2) (AA 1-417) protein (His tag)
PGK2 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Phosphoglycerate Kinase 2 (PGK2) (AA 1-417) protein (GST tag)PGK2 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Phosphoglycerate Kinase 2 (PGK2) (AA 268-339) protein (GST tag)PGK2 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Phosphoglycerate Kinase 2 (PGK2) (AA 1-417) protein (Strep Tag)Crystallography grade PGK2 Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA
Phosphoglycerate Kinase 2 (PGK2) (AA 2-417) protein (His tag)Crystallography grade PGK2 Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Phosphoglycerate Kinase 2 (PGK2) protein (Myc-DYKDDDDK Tag)PGK2 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
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Bioactivity Validated
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.5 mg/mL
- Buffer
- Liquid. 20 mM Tris-HCl buffer ( pH 8.0) containing 20 % glycerol, 1 mM DTT, 0.1M NaCl
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- Target
- PGK2 (Phosphoglycerate Kinase 2 (PGK2))
- Alternative Name
- PGK2 (PGK2 Products)
- Synonyms
- PGKB Protein, PGKPS Protein, dJ417L20.2 Protein, Pgk-2 Protein, Tcp-2 Protein, PGK2 Protein, phosphoglycerate kinase 2 Protein, PGK2 Protein, Pgk2 Protein, cbbK Protein
- Background
- PGK2 is a testis-specific form of phosphoglycerate kinase. Initially assumed to be a pseudogene, this protein is actually a functional phosphoglycerate kinase that catalyzes the reversible conversion of 1,3-bisphosphoglycerate to 3-phosphoglycerate, during the Embden-Meyerhof-Parnas pathway of glycolysis, in the later stages of spermatogenesis. Recombinant human PGK2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.
- Molecular Weight
- 46.9 kDa (437aa), confirmed by MALDI-TOF
- NCBI Accession
- NP_620061
- UniProt
- P07205
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