MGLL Protein (AA 1-313) (His tag)
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- Target See all MGLL Proteins
- MGLL (Monoglyceride Lipase (MGLL))
- Protein Type
- Recombinant
- Biological Activity
- Active
- Protein Characteristics
- AA 1-313
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This MGLL protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Sequence
- MGSSHHHHHH SSGLVPRGSH MTTSASSHLN KGIKQVYMSL PQGEKVQAMY IWIDGTGEGL RCKTRTLDSE PKCVEELPEW NFDGSSTLQS EGSNSDMYLV PAAMFRDPFR KDPNKLVLCE VFKYNRRPAE TNLRHTCKRI MDMVSNQHPW FGMEQEYTLM GTDGHPFGWP SNGFPGPQGP YYCGVGADRA YGRDIVEAHY RACLYAGVKI AGTNAEVMPA QWEFQIGPCE GISMGDHLWV ARFILHRVCE DFGVIATFDP KPIPGNWNGA GCHTNFSTKA MREENGLKYI EEAIEKLSKR HQYHIRAYDP KGGLDNARRL TGFHETSNIN DFSAGVANRS ASIRIPRTVG QEKKGYFEDR RPSANCDPFS VTEALIRTCL LNETGDEPFQ YKN
- Purity
- > 90 % by SDS - PAGE
- Biological Activity Comment
- Specific activity is >2,800 pmol/min/ug, and is defined as the amount of enzyme that convert 1.0 pmole of L-glutamate to L-glutamine per miunte at pH 7.5 at 37C in coupled system with PK/LDH.
- Top Product
- Discover our top product MGLL Protein
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Monoglyceride Lipase (MGLL) (AA 1-313) protein (GST tag)
MGLL Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Monoglyceride Lipase (MGLL) (AA 1-333) protein (His tag)MGLL Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Monoglyceride Lipase (MGLL) (AA 1-313) protein (His tag)MGLL Origin: Human Host: Escherichia coli (E. coli) Recombinant > 85 % by SDS - PAGE SDS
Monoglyceride Lipase (MGLL) (AA 1-303) protein (Strep Tag)Crystallography grade MGLL Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA
Monoglyceride Lipase (MGLL) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)MGLL Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
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Bioactivity Validated
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- Liquid. In 20 mM Tris-HCl buffer ( pH 8.0) containing 20 % glycerol, 5 mM DTT, 200 mM NaCl
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- Target
- MGLL (Monoglyceride Lipase (MGLL))
- Alternative Name
- MGLL (MGLL Products)
- Synonyms
- cb909 Protein, wu:fc66g01 Protein, zgc:56561 Protein, MGC84195 Protein, MGLL Protein, mgl Protein, hu-k5 Protein, mgll Protein, HU-K5 Protein, HUK5 Protein, MAGL Protein, MGL Protein, AA589436 Protein, Magl Protein, Mgl Protein, monoglyceride lipase Protein, monoglyceride lipase L homeolog Protein, 13L protein Protein, Monoglyceride lipase Protein, mgll Protein, MGLL Protein, mgll.L Protein, 13L Protein, YMTVg13L Protein, EHI_044210 Protein, EHI_045330 Protein, TATV_DAH68_039 Protein, Mgll Protein
- Background
- Glutamine synthetase (GLUL), which is therefore able to regulate intracellular concentrations of glutamate. GLUL catalyzes the synthesis of glutamine from glutamate and ammonia. Glutamine is a main source of energy and is involved in cell proliferation, inhibition of apoptosis, and cell signaling. GLUL is essential for proliferation of fetal skin fibroblasts and plays an important role in controlling body pH by removing ammonia from circulation. Mutations in GLUL are associated with congenital glutamine deficiency. Recombinant GLUL protein , fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
- Molecular Weight
- 44.2kDa (393aa), confirmed by MALDI-TOF
- NCBI Accession
- NP_001028216
- UniProt
- P15104
- Pathways
- Regulation of G-Protein Coupled Receptor Protein Signaling, Lipid Metabolism
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