ADAM10 Protein (AA 214-672) (His tag)

Catalog No. ABIN6387898

Product Details

Target: ADAM10 (ADAM Metallopeptidase Domain 10 (ADAM10))

Protein Type: Recombinant

Protein Characteristics: AA 214-672

Origin: Human

Source: Baculovirus infected Insect Cells

Purification tag / Conjugate: This ADAM10 protein is labelled with His tag.

Application: SDS-PAGE (SDS)

Sequence: TTSAEKNTCQ LYIQTDHLFF KYYGTREAVI AQISSHVKAI DTIYQTTDFS GIRNISFMVK RIRINTTADE KDPTNPFRFP NIGVEKFLEL NSEQNHDDYC LAYVFTDRDF DDGVLGLAWV GAPSGSSGGI CEKSKLYSDG KKKSLNTGII TVQNYGSHVP PKVSHITFAH EVGHNFGSPH DSGTECTPGE SKNLGQKENG NYIMYARATS GDKLNNNKFS LCSIRNISQV LEKKRNNCFV ESGQPICGNG MVEQGEECDC GYSDQCKDEC CFDANQPEGR KCKLKPGKQC SPSQGPCCTA QCAFKSKSEK CRDDSDCARE GICNGFTALC PASDPKPNFT DCNRHTQVCI NGQCAGSICE KYGLEECTCA SSDGKDDKEL CHVCCMKKMD PSTCASTGSV QWSRHFSGRT ITLQPGSPCN DFRGYCDVFM RCRLVDADGP LARLKKAIFS PELYENIAEL EHHHHHH

Purity: > 85 % by SDS - PAGE

Endotoxin Level: < 1.0 EU per 1 microgram of protein (determined by LAL method)

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Liquid. In Phosphate Buffered Saline (  pH 7.4) containing 10 % glycerol.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

Target Details

Target: ADAM10 (ADAM Metallopeptidase Domain 10 (ADAM10))

Alternative Name: ADAM10 (ADAM10 Products)

Synonyms: ADAM10 Protein, Adam10 Protein, adam10 Protein, wu:fc03d12 Protein, ad10 Protein, cd156c Protein, kuz Protein, kuzbanian Protein, madm Protein, xadam10 Protein, ADAM-10 Protein, ADAM 10 Protein, AD10 Protein, CD156c Protein, HsT18717 Protein, MADM Protein, 1700031C13Rik Protein, LOC100219653 Protein, zgc:64203 Protein, ADAM metallopeptidase domain 10 Protein, ADAM metallopeptidase domain 10a Protein, ADAM metallopeptidase domain 10 S homeolog Protein, a disintegrin and metallopeptidase domain 10 Protein, ADAM metallopeptidase domain 10b Protein, ADAM10 Protein, Adam10 Protein, adam10a Protein, adam10.S Protein, adam10b Protein

Background: ADAM10, as known as disintegrin and metalloproteinase domain-containing protein 10 isoform 1, is a member of the ADAM family that contains a disintegrin and metallogrotease-like domain. This protein is a sheddase, and has a broad specificity for peptide hydrolysis reactions. Also, it cleaves ephrin, within the ephrin/eph complex, formed between two cell surfaces. When ephrin is freed from the opposing cell, the entire ephrin/eph complex is endocytosed. In neurons, ADAM10 is the most important enzyme with alpha-secretase activity for proteolytic processing of the amyloid precursor protein. Recombinant human ADAM10, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Molecular Weight: 51.6kDa (467aa) 50-70kDa (SDS-PAGE under reducing conditions.)

NCBI Accession: NP_001101

UniProt: O14672

Pathways: Notch Signaling, EGFR Signaling Pathway