Adenylate Kinase 2 Protein (AK2) (AA 1-239) (His tag)
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- Target See all Adenylate Kinase 2 (AK2) Proteins
- Adenylate Kinase 2 (AK2)
- Protein Type
- Recombinant
- Biological Activity
- Active
- Protein Characteristics
- AA 1-239
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Origin
- Mouse
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This Adenylate Kinase 2 protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Sequence
- MGSSHHHHHH SSGLVPRGSH MGSHMAPNVL ASEPEIPKGI RAVLLGPPGA GKGTQAPKLA ENFCVCHLAT GDMLRAMVAS GSELGKKLKA TMDAGKLVSD EMVVELIEKN LETPSCKNGF LLDGFPRTVR QAEMLDDLME KRKEKLDSVI EFSIQDSLLI RRITGRLIHP KSGRSYHEEF NPPKEPMKDD ITGEPLIRRS DDNEKALKTR LEAYHTQTTP LVEYYRKRGI HCAIDASQTP DIVFASILAA FSKATCKDLV MFI
- Purity
- > 95 % by SDS - PAGE
- Biological Activity Comment
- Specific activity is > 40 units/mg. One unit will convert 2.0 umoles of ADP to ATP + AMP per minute at pH 7.5 at 37C.
- Top Product
- Discover our top product AK2 Protein
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Adenylate Kinase 2 (AK2) (AA 1-239) protein (His tag)
AK2 Origin: Mouse Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
Adenylate Kinase 2 (AK2) (AA 1-239) protein (His tag)AK2 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
Adenylate Kinase 2 (AK2) (AA 1-239) protein (GST tag)AK2 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
Adenylate Kinase 2 (AK2) (AA 1-96) protein (GST tag)AK2 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
Adenylate Kinase 2 (AK2) protein (GST tag,His tag)AK2 Origin: Human Host: Baculovirus infected Insect Cells Recombinant > 95 % as determined by reducing SDS-PAGE.
Adenylate Kinase 2 (AK2) (AA 1-239) protein (Strep Tag)Crystallography grade AK2 Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
Adenylate Kinase 2 (AK2) (AA 2-239) protein (His tag)Crystallography grade AK2 Origin: Human Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS, Crys
Adenylate Kinase 2 (AK2) (AA 2-239) protein (His tag)Crystallography grade AK2 Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS, Crys
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
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Bioactivity Validated
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.5 mg/mL
- Buffer
- Liquid. In 20 mM Tris-Hcl buffer ( pH 8.5) containing 10 % glycerol, 1 mM DTT
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- Target
- Adenylate Kinase 2 (AK2)
- Alternative Name
- Ak2 (AK2 Products)
- Synonyms
- ADK-2 Protein, BcDNA:SD09634 Protein, CG3140 Protein, Dak2 Protein, Dmel\\CG3140 Protein, anon-Dak2 Protein, adk2 Protein, NV10896 Protein, wu:fb34f05 Protein, wu:fj80e03 Protein, ADK2 Protein, AK 2 Protein, Ak-2 Protein, D4Ertd220e Protein, MXI22.8 Protein, MXI22_8 Protein, Adenylate kinase 2 Protein, adenylate kinase 2 Protein, adenylate kinase Protein, adenylate kinase 2 S homeolog Protein, Adenylate kinase family protein Protein, Adk2 Protein, AK2 Protein, ak2 Protein, LOC100123971 Protein, ak2.S Protein, Ak2 Protein, AT5G50370 Protein
- Background
- Ak2, also known as Adenylate kinase 2 mitochondrial isoform a, is a ubiquitous monomeric enzyme involved energy metabolism of prokaryotic and eukaryotic cells. Three isozymes (Ak1, Ak2 and Ak3) are characterized in vertebrates. Expression of these isozymes is tissue-specific and developmentally regulated. Ak2 is localized in the mitochondrial intermembrane space and may pay a role in apoptosis.Recombinant mouse Ak2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
- Molecular Weight
- 29.0 kDa (263aa) confirmed by MALDI-TOF
- NCBI Accession
- NP_001029138
- UniProt
- Q9WTP6
- Pathways
- Nucleotide Phosphorylation, Ribonucleoside Biosynthetic Process
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