NEDD8 Protein (AA 1-76) (His tag)
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- Target See all NEDD8 Proteins
- NEDD8 (Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-76
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This NEDD8 protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Characteristics
- NEDD8, 1-76 aa, Human, His tagged, Recombinant, E.coli
- Purity
- > 95 % by SDS - PAGE
- Top Product
- Discover our top product NEDD8 Protein
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- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.5 mg/ml (determined by Bradford assay)
- Buffer
- In 20 mM Tris-HCl buffer (pH 8.0) containing 0.5mM DTT, 50mM NaCl, 10% glycerol
- Storage
- 4 °C
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- Target
- NEDD8 (Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8))
- Alternative Name
- NEDD8 (NEDD8 Products)
- Synonyms
- NEDD-8 Protein, Rub1 Protein, CG10679 Protein, Dmel\\CG10679 Protein, NEDD8 Protein, dNEDD8 Protein, nedd8 Protein, ubiquitin Protein, si:zc14a17.5 Protein, GB17785 Protein, DDBDRAFT_0218116 Protein, DDBDRAFT_0238041 Protein, DDB_0218116 Protein, DDB_0238041 Protein, nedd8.S Protein, neural precursor cell expressed, developmentally down-regulated 8 Protein, neural precursor cell expressed, developmentally down-regulated gene 8 Protein, CG10679 gene product from transcript CG10679-RB Protein, Nedd8 Protein, ubiquitin-like protein Nedd8 Protein, neural precursor cell expressed, developmentally down-regulated 8 L homeolog Protein, NEDD8 Protein, Nedd8 Protein, nedd8 Protein, nedd8.L Protein
- Background
- NEDD8 belongs to the ubiquitin family and human NEDD8 shares 60% amino acid sequence identity to ubiquitin. The NEDD8 system is essential for the regulation of protein degradation pathways involved in cell cycle progression, morphogenesis and tumorigenesis. Attachment of NEDD8 to cullins activates their associated E3 ubiquitin ligase activity, and thus promotes polyubiquitination and proteasomal degradation of cyclins and other regulatory proteins. Recombinant human NEDD8 protein was expressed in E.coli and purified by using conventional chromatography techniques, after refolding of the isolated inclusion body in renaturation buffer. Synonyms: Ubiquitin-like protein Nedd8, Neddylin, Neural precursor cell expressed, developmentally down-regulated 8 MGC104393, MGC125896, MGC125897, NED8, 0NEDD 8, Neddylin, Neural precursor cell expressed developmentally down regulated 8, Neural precursor cell expressed developmentally down regulated gene 8, Rub1, Ubiquitin like protein Nedd 8, Ubiquitin like protein Nedd8. NCBI no.: NP_006147
- Molecular Weight
- 12.8 kDa (113 aa), confirmed by MALDI-TOF.
- Pathways
- Ubiquitin Proteasome Pathway
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