DFFA Protein (AA 1-331) (His tag)
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- Target See all DFFA Proteins
- DFFA (DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-331
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This DFFA protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Characteristics
- DFFA, 1-331 aa, Human, His-tagged, Recombinant, E.coli
- Purity
- > 90 % by SDS-PAGE
- Top Product
- Discover our top product DFFA Protein
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- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/ml (determined by Bradford assay)
- Buffer
- Liquid. In 20mM Tris-HCl buffer(pH 8.0) containing 10% glycerol 1mM DTT.
- Storage
- 4 °C
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- Target
- DFFA (DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA))
- Alternative Name
- DFFA (DFFA Products)
- Synonyms
- DFF-45 Protein, DFF1 Protein, ICAD Protein, A330085O09Rik Protein, DFF35 Protein, Dff45 Protein, ICAD-L Protein, ICAD-S Protein, DFFA Protein, zgc:92229 Protein, DNA fragmentation factor subunit alpha Protein, DNA fragmentation factor, alpha subunit Protein, DNA fragmentation factor, 45kDa, alpha polypeptide Protein, DNA fragmentation factor, alpha polypeptide Protein, DFFA Protein, Dffa Protein, dffa Protein
- Background
- DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA, also known as DNA fragmentation factor subunit alpha, is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Recombinant DFFA protein was expressed in E.coli and purified by using conventional chromatography techniques. Synonyms: FF-45, DFF1, ICAD, DNA fragmentation factor subunit alpha, A330085O09Rik, ICAD L, ICAD S, DFF 1, Caspase activated deoxyribonuclease inhibitor short form, DFF 45, DFF alpha, DFF35, DFF45, DFFA, Caspase-activated deoxyribonuclease inhibitor short form, Dffa DNA fragmentation factor, alpha subunit, DNA fragmentation factor 45 kDa subunit, DNA Fragmentation Factor Alpha Subunit, DNA fragmentation factor subunit alpha, DNA fragmentation factor, 45 kD, alpha subunit, DNA fragmentation factor, 45kDa, alpha polypeptide, DNA fragmentation factor, 45kDa, alpha polypeptide (DFFA), transcript variant 1, RP23 121D17.3, DNA fragmentation factor, alpha subunit, DNAation factor 45 kDa subunit, H13, Inhibitor of CAD, Inhibitor of Caspase Activated DNase, MGC143066, OTTHUMP00000001903, OTTHUMP00000001904,. NCBI no.: AAH07721
- Molecular Weight
- 38.7 kDa (351aa), confirmed by MALDI-TOF.
- Pathways
- Apoptosis, Caspase Cascade in Apoptosis
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