IL-1 beta Protein

Catalog No. ABIN6699988

Supplier Product No.: 009-001-301

This Recombinant IL-1 beta protein is produced in Escherichia coli (E. coli).

Quick Overview for IL-1 beta Protein (ABIN6699988)

Target: IL-1 beta (IL1B) (Interleukin 1, beta (IL1B))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Purity: 97,00%

Product Details

Supplier Product No.: 009-001-301

Supplier: Rockland

Purpose: IL-1ß Human Recombinant Protein

Purification: Recombinant protein corresponding to amino acids 1 to 153 of mature human Interleukin-1beta (IL-1β). Purity is greater than 97% as determined by reducing and non-reducing SDS-PAGE and by analytical RP-HPLC, each against a known standard.

Endotoxin Level: Measured by LAL is typically ≤ 1 EU/μg protein.

Biological Activity Comment: IL-1ß activity is determined by the stimulation of D10S.G4.1 cells and is typically less than 12 pg/mL.

Application Details

Application Notes:

Application Note: Interleukin-1beta (IL-1β) has been tested by SDS-PAGE and is suitable as a control for polyclonal or monoclonal anti-Interleukin-1beta (IL-1β) in immunological assays.

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 10 μL (10-100 μL)

Concentration: 0.1 mg/mL

Buffer:

Buffer: 0.01 M Sodium Phosphate, pH 7.5

Stabilizer: None

Preservative: Without preservative

Storage: -20 °C

Storage Comment: Store vial at -20° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL) . For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.

Expiry Date: 6 months

Target Details

Target: IL-1 beta (IL1B) (Interleukin 1, beta (IL1B))

Alternative Name: IL1B

Background:

Synonyms: IL-1b cytokine, Interleukin 1b, IL-1ß, IL-1 beta, Catabolin, H1, Hematopoietin 1, IFN beta inducing factor, Interleukin 1 beta, LAF, OAF, Osteoclast activating factor

Background: Interleukin-1beta (IL-1β) is a proinflammatory cytokine produced in response to inflammatory agents by a variety of cells, including monocytes, macrophages, and dendric cells (DCs). IL-1β and IL-1α are two distinct and independently regulated gene products that comprise IL-1 and signal through the Type 1 IL-1 receptor (IL-1R1). Although IL-1α is cell associated and IL-1β is secreted, they have nearly identical biological activity in that they induce adhesion molecule expression on epithelial cells, control fever induction, and play a role in arthritis and septic shock. Signaling activated by the IL-1R1 promotes these activities through a MYD88 signaling pathway similar to those associated with Toll receptors. Recombinant human IL-1β is a non-glycosylated single chain 17,376 Da protein containing 153 amino acid residues.

UniProt: P01584

Pathways: NF-kappaB Signaling, Interferon-gamma Pathway, TLR Signaling, Negative Regulation of Hormone Secretion, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Autophagy, Cancer Immune Checkpoints, Inflammasome