IL-1 beta Protein

Catalog No. ABIN6699993

Supplier Product No.: 009-f01-v25-0100

Recombinant IL-1 beta protein expressed in Escherichia coli (E. coli).

Quick Overview for IL-1 beta Protein (ABIN6699993)

Target: IL-1 beta (IL1B) (Interleukin 1, beta (IL1B))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Purity: 97,00%

Grade: Animal-Free

Product Details

Supplier Product No.: 009-f01-v25-0100

Supplier: Rockland

Purpose: Human Interleukin-1-beta Recombinant Protein (Animal Free)

Purification: Interleukin-1 beta is produced with no animal-derived raw products, animal free equipment and animal free protocols. Purity was determined to be greater than 97% as determined by HPLC, analysis by UV-Spectroscopy at 280nm, and by reducing and non-reducing SDS-PAGE.

Endotoxin Level: Measured by LAL is typically ≤ 1 EU/μg protein.

Biological Activity Comment: The activity is determined by the stimulation of D10S.G4.1 cells and is typically less than 12 pg/mL.

Application Details

Application Notes:

Application Note: Interleukin-1-beta Recombinant Protein is suitable as a control for polyclonal or monoclonal anti-Interleukin-1-beta in immunological assays.

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 100 μL

Buffer:

Buffer: 0.01 M Sodium Phosphate, pH 7.5

Stabilizer: None

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.

Expiry Date: 6 months

Target Details

Target: IL-1 beta (IL1B) (Interleukin 1, beta (IL1B))

Alternative Name: IL1B

Background:

Synonyms: Catabolin, LAF, EP, LEM, MCF

Background: Interleukin-1 beta (IL-1β) is a pro-inflammatory cytokine produced in response to inflammatory agents by a variety of cells, including monocytes, macrophages, and dendritic cells (DCs). IL-1β and IL-1α are two distinct and independently regulated gene products that comprise IL-1 and signal through the Type 1 IL-1 receptor (IL-1R1). Although IL-1α is cell associated and IL-1β is secreted, they have nearly identical biological activity in that they induce adhesion molecule expression on epithelial cells, control fever induction, and play a role in arthritis and septic shock. Signaling activated by the IL-1R1 promotes these activities through a MYD88 signaling pathway similar to those associated with Toll receptors. Recombinant human IL-1β is a non-glycosylated protein, containing 153 amino acids, with a molecular weight of 17.4 kDa.

Pathways: NF-kappaB Signaling, Interferon-gamma Pathway, TLR Signaling, Negative Regulation of Hormone Secretion, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Autophagy, Cancer Immune Checkpoints, Inflammasome