CXCL12 Protein (AA 24-89) (Biotin)

Catalog No. ABIN6952223

Product Details

Target: CXCL12 (Chemokine (C-X-C Motif) Ligand 12 (CXCL12))

Protein Type: Recombinant

Biological Activity: Active

Protein Characteristics: AA 24-89

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This CXCL12 protein is labelled with Biotin.

Application: Flow Cytometry (FACS), Cell Migration Assay (CMA)

Sequence: KPVSLSYRCP CRFFESHVAR ANVKHLKILN TPNCALQIVA RLKNNNRQVC IDPKLKWIQE YLEKALNK

Characteristics: Biotininylated Recombinant Human CXCL12 (SDF-1alpha)

Purity: > 97 % by SDS PAGE

Endotoxin Level: <0.01 EU per 1μg of the protein by the LAL method

Biological Activity Comment: EC50 = 0.5-0.9 nM determined by Calcium Flux with human CXCR4 in U937 cells
Migration confirmed with U937 cells expressing CXCR4

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Spin tube prior to resuspending. Recommended at 100 μg/mL in sterile water

Storage: -20 °C,-80 °C

Storage Comment: 12 months from date of receipt, -20 to -70 °C as supplied.
Suggest to use immediately after reconstitution
1 month at -20 to -70 °C under sterile conditions after reconstitution.

Expiry Date: 12 months

Target Details

Target: CXCL12 (Chemokine (C-X-C Motif) Ligand 12 (CXCL12))

Alternative Name: CXCL12/SDF-1alpha (CXCL12 Products)

Synonyms: IRH Protein, PBSF Protein, SCYB12 Protein, SDF1 Protein, TLSF Protein, TPAR1 Protein, CINC-1 Protein, Gro1 Protein, Pbsf Protein, Scyb12 Protein, Sdf1 Protein, Tlsf Protein, Tpar1 Protein, Fsp Protein, KC Protein, Mgsa Protein, N51 Protein, Scyb1 Protein, gro Protein, sdf-1 Protein, sdf1 Protein, xSDF-1 Protein, SDF-1 Protein, cxcl12 Protein, sdf-1a Protein, sdf1a Protein, wu:fa55e10 Protein, wu:fc16h12 Protein, wu:fj84c02 Protein, C-X-C motif chemokine ligand 12 Protein, C-X-C motif chemokine ligand 1 Protein, chemokine (C-X-C motif) ligand 12 Protein, chemokine (C-X-C motif) ligand 1 Protein, C-X-C motif chemokine ligand 12 L homeolog Protein, chemokine (C-X-C motif) ligand 12a (stromal cell-derived factor 1) Protein, CXCL12 Protein, cxcl12 Protein, Cxcl1 Protein, Cxcl12 Protein, cxcl12.L Protein, cxcl12a Protein

Background: Stromal-Cell Derived Factor-1alpha (SDF-1alpha) (CXCL12) attracts lymphocytes and plays important roles in embryogenesis and angiogenesis with implications in tumor metastasis. By binding to CXCR4, one of the co-receptors for HIV viral entry, SDF-1alpha can suppress HIV. Besides CXCR4, SDF-1alpha also binds to the “decoy” receptor CXCR7, and activates the downstream beta-arrestin- rather than G protein-mediated signaling pathway.

Molecular Weight: 10.381 kDa

UniProt: P48061

Pathways: Regulation of Cell Size, CXCR4-mediated Signaling Events, Negative Regulation of intrinsic apoptotic Signaling