SARS-CoV-2 NSP7, NSP8 protein (His tag)

Catalog No. ABIN6952711

Product Details

Target: SARS-CoV-2 NSP7, NSP8

Protein Type: Recombinant

Origin: SARS Coronavirus-2 (SARS-CoV-2)

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: His tag

Purpose: SARS-CoV-2 (COVID-19) NSP7&NSP8 Protein, His Tag

Sequence: AA 1-83

Characteristics: SARS-CoV-2 NSP7&NSP8, His Tag (NS8-C5125) is expressed from E.coli cells. It contains AA Ser 1 - Gln 83 (NSP7) & Ala 1 - Gln 198 (NSP8) (Accession # YP_009725303.1 (NSP7) & YP_009725304.1 (NSP8). Predicted N-terminus: Met
This protein carries a polyhistidine tag.

Purity: >95 % as determined by SDS-PAGE.

Endotoxin Level: Less than 1.0 EU per μg by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS, pH 7.4

Storage: -80 °C

Storage Comment: The product MUST be stored at -70°C or lower upon receipt
-70°C for 3 months under sterile conditions.

Target Details

Target: SARS-CoV-2 NSP7, NSP8

Alternative Name: SARS-CoV-2 NSP7 & NSP8

Target Type: Viral Protein

Background: During the formation of the coronaviral replication/transcription complex, essential steps include processing of the conserved polyprotein nsp7-10 region by the main protease Mpro and subsequent complex formation of the released nsp's. Upon infecting host cells, coronaviruses assemble a multi-subunit RNA-synthesis complex of viral non-structural proteins (nsp) responsible for the replication and transcription of the viral genome. non-structural proteins 7 (NSP7) forms a hexadecamer with nsp8 (8 subunits of each) that may participate in viral replication by acting as a primase. Alternatively, may synthesize substantially longer products than oligonucleotide primers.

Molecular Weight: 32.1 kDa