EPH Receptor A3 Protein (EPHA3) (AA 21-541) (His tag,mFc Tag)

Catalog No. ABIN6961115

Recombinant EPH Receptor A3 protein expressed in HEK-293 Cells.

Quick Overview for EPH Receptor A3 Protein (EPHA3) (AA 21-541) (His tag,mFc Tag) (ABIN6961115)

Target: EPH Receptor A3 (EPHA3)

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Affinity Measurement (AM), Antibody Drug Assay (ADA), Immunoassay (IA), Screening Assay (ScA)

Purity: The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.

Product Details

Protein Characteristics: AA 21-541

Purification tag / Conjugate: This EPH Receptor A3 protein is labelled with His tag,mFc Tag.

Purpose: Recombinant human EPHA3 protein with C-terminal mouse Fc and 6xHis tag

Specificity: EPHA3(Glu21-Gln541) mFc(Pro99-Lys330) 6xHis tag

Characteristics: Extracellular Domain Protein

Purification: Purified from cell culture supernatant by affinity chromatography

Application Details

Application Notes: Extracellular Domain Proteins (ECD) can be used as:

• Immunogens for antibody drug development
• Reagents used for CAR-T positive cell monitoring
• Reagents for antibody screening and functional testing
• Reagents for antibody affinity measurement

Comment:

The protein was produced using HEK293 mammalian cell secretion expression system to ensure the close-to-native structures and post-translational modifications of the target protein.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please see Certificate of Analysis for specific instructions of reconstitution.

Buffer: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose is added as protectants before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Expiry Date: 12 months

Target Details

Target: EPH Receptor A3 (EPHA3)

Alternative Name: EPHA3

Background: TYRO4,HEK4,ETK1,ETK,EK4,HEK
This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. This gene encodes a protein that binds ephrin-A ligands. Two alternatively spliced transcript variants have been described for this gene.

Molecular Weight: predicted molecular mass of 85.9 kDa after removal of the signal peptide.

Gene ID: 2042

UniProt: P29320

Pathways: RTK Signaling, Regulation of Cell Size