LY9 Protein (AA 48-454) (His tag,AVI tag,Biotin)

Catalog No. ABIN6972974

Product Details

Target: LY9 (CD229) (Lymphocyte Antigen 9 (CD229))

Protein Type: Recombinant

Biological Activity: Active

Protein Characteristics: AA 48-454

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This LY9 protein is labelled with His tag,AVI tag,Biotin.

Specificity: Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

Characteristics: Biotinylated Human CD229 / SLAMF3 Protein, His,Avitag™

Purity: >95 % as determined by SDS-PAGE.

Endotoxin Level: Less than 1.0 EU per μg by the LAL method.

Application Details

Comment:

Ready-to-use Avitag™ biotinylated protein:
The product is exclusively produced using the Avitag™ technology. Briefly, a unique 15 amino acid peptide, the Avi tag, is introduced into the recombinant protein during expression vector construction. The single lysine residue in the Avi tag is enzymatically biotinylated by the E. Coli biotin ligase BirA.

This single-point enzymatic labeling technique brings many advantages for commonly used binding assays. The biotinylation happens on the lysine residue of Avi tag, and therefore does NOT interfere with the target protein's natural binding activities. In addition, when immobilized on an avidin-coated surface, the protein orientation is uniform because the position of the Avi tag in the protein is precisely controlled.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: PBS, pH 7.4

Storage: -20 °C

Target Details

Target: LY9 (CD229) (Lymphocyte Antigen 9 (CD229))

Alternative Name: CD229 (CD229 Products)

Synonyms: LY9 Protein, CD229 Protein, SLAMF3 Protein, hly9 Protein, mLY9 Protein, AI893573 Protein, Lgp100 Protein, Ly-9 Protein, T100 Protein, lymphocyte antigen 9 Protein, LY9 Protein, Ly9 Protein

Background: CD229, also known as Ly9 and SLAMF3, is a 120  kDa type I transmembrane glycoprotein in the SLAM subgroup of the CD2 family. Signaling lymphocyte activation molecule (SLAM) family receptors are critically involved in modulating innate and adaptive immune responses. CD229 is expressed on T and B cells, thymocytes, and more weakly on NK cells. Homophilic binding between CD229 Molecules is mediated by the N-terminal Ig-like domain.

Molecular Weight: 48.5 kDa

NCBI Accession: NP_002339

Pathways: BCR Signaling