TNFRSF1B Protein (AA 23-257) (His tag,AVI tag,Biotin)

Catalog No. ABIN6973283

Product Details

Target: TNFRSF1B (Tumor Necrosis Factor Receptor Superfamily, Member 1B (TNFRSF1B))

Protein Type: Recombinant

Biological Activity: Active

Protein Characteristics: AA 23-257

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This TNFRSF1B protein is labelled with His tag,AVI tag,Biotin.

Sequence: AA 23-257

Specificity: Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

Characteristics: Human TNFSF11 / RANKL / CD254 Protein, Mouse IgG2a Fc Tag

Purity: >90 % as determined by SDS-PAGE.

Endotoxin Level: Less than 1.0 EU per μg by the LAL method.

Application Details

Comment:

Ready-to-use Avitag™ biotinylated protein:
The product is exclusively produced using the Avitag™ technology. Briefly, a unique 15 amino acid peptide, the Avi tag, is introduced into the recombinant protein during expression vector construction. The single lysine residue in the Avi tag is enzymatically biotinylated by the E. Coli biotin ligase BirA.

This single-point enzymatic labeling technique brings many advantages for commonly used binding assays. The biotinylation happens on the lysine residue of Avi tag, and therefore does NOT interfere with the target protein's natural binding activities. In addition, when immobilized on an avidin-coated surface, the protein orientation is uniform because the position of the Avi tag in the protein is precisely controlled.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: PBS, pH 7.4

Storage: -20 °C

Target Details

Target: TNFRSF1B (Tumor Necrosis Factor Receptor Superfamily, Member 1B (TNFRSF1B))

Alternative Name: TNFR2 (TNFRSF1B Products)

Synonyms: CD120b Protein, TBPII Protein, TNF-R-II Protein, TNF-R75 Protein, TNFBR Protein, TNFR1B Protein, TNFR2 Protein, TNFR80 Protein, p75 Protein, p75TNFR Protein, TNF-RII/TNFRSF1B Protein, TNFR-II Protein, TNFRSF1B Protein, TNF-R2 Protein, TNF-alphaR2 Protein, TNFRII Protein, TNFalpha-R2 Protein, Tnfr-1 Protein, Tnfr2 Protein, rank Protein, zgc:163064 Protein, TNF receptor superfamily member 1B Protein, tumor necrosis factor receptor superfamily, member 1b Protein, tumor necrosis factor receptor superfamily, member 1B Protein, TNFRSF1B Protein, Tnfrsf1b Protein, tnfrsf1b Protein

Background: TNF RI is also known as the p60 or p55 TNFR) and TNF RII (the p75 or p80 TNFR) are two distinct type I transmembrane glycoproteins that bind TNF with highaffinity.Both RI and RII are prototypic members of the TNF receptor superfamily and have been designated TNFRSF1A and TNFRSF1B, respectively. Human TNF RII cDNA encodes a 461 amino acid (aa) residue precursor protein with a 22 aa putative signal peptide, a 235 aa extracellular domain, a 20 aa transmembrane domain and a 174 aa cytoplasmic domain. TNFRII is expressed in fetal brain. The protein is produced naturally as a soluble form (sTNFRII). The soluble receptor inhibits TNFα action by competing with cell surface receptors in binding TNFα, thereby blocking its biologic effects. TNFRII is strongly expressed at the cartilage-pannus junction, and plays a major role in a subset of families with multiple cases of rheumatoid arthritis (RA). Further, high plasma levels of sTNFRII were significantly associated with increased incidence of coronary heart disease, independent of established cardiovascular risk factors, and seems to be useful for monitoring the inflammatory activity of sarcoidosis.

Molecular Weight: 28.7 kDa

Pathways: NF-kappaB Signaling, Apoptosis, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Ubiquitin Proteasome Pathway