GUCY2D Protein (His-Avi Tag,Biotin)

Catalog No. ABIN7199197

Product Details

Target: GUCY2D (Guanylate Cyclase 2D, Membrane (Retina-Specific) (GUCY2D))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This GUCY2D protein is labelled with His-Avi Tag,Biotin.

Purpose: Biotinylated Human GUCY2D / Retinal guanylyl cyclase 1 Protein, His,Avitag™

Sequence: Ala 52 - Glu 462

Characteristics: Biotinylated Human GUCY2D, His,Avitag is expressed from human 293 cells (HEK293). It contains AA Ala 52 - Glu 462 (Accession # Q02846-1).

Purity: >95 % as determined by SDS-PAGE.

Endotoxin Level: Less than 1.0 EU per μg by the LAL method.

Application Details

Application Notes: This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™). The protein has a calculated MW of 46.7 kDa. The protein migrates as 50-55 kDa under reducing (R) condition due to glycosylation.

Comment:

Ready-to-use Avitag™ biotinylated protein:
The product is exclusively produced using the Avitag™ technology. Briefly, a unique 15 amino acid peptide, the Avi tag, is introduced into the recombinant protein during expression vector construction. The single lysine residue in the Avi tag is enzymatically biotinylated by the E. Coli biotin ligase BirA.

This single-point enzymatic labeling technique brings many advantages for commonly used binding assays. The biotinylation happens on the lysine residue of Avi tag, and therefore does NOT interfere with the target protein's natural binding activities. In addition, when immobilized on an avidin-coated surface, the protein orientation is uniform because the position of the Avi tag in the protein is precisely controlled.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: PBS, pH 7.4

Storage: -20 °C

Storage Comment: -20°C

Target Details

Target: GUCY2D (Guanylate Cyclase 2D, Membrane (Retina-Specific) (GUCY2D))

Alternative Name: GUCY2D / Retinal guanylyl cyclase 1 (GUCY2D Products)

Synonyms: GUCY2D Protein, CORD5 Protein, CORD6 Protein, CYGD Protein, GUC1A4 Protein, GUC2D Protein, LCA Protein, LCA1 Protein, RCD2 Protein, RETGC-1 Protein, ROS-GC1 Protein, ROSGC Protein, retGC Protein, guanylate cyclase 2D, retinal Protein, guanylate cyclase 2d Protein, GUCY2D Protein, Gucy2d Protein

Background: Synonyms: GUCY2D,Retinal guanylyl cyclase 1,RETGC-1,CORD6,GUC1A4,GUC2D,ROS-GC,
GUCY2D also known as CORD6, GUC1A4, GUC2D ROS-GC and RETGC-1, belongs to the adenylyl cyclase class-4/guanylyl cyclase family. Catalyzes the synthesis of cyclic GMP (cGMP) in rods and cones of photoreceptors. Plays an essential role in phototransduction, by mediating cGMP replenishment. May also participate in the trafficking of membrane-asociated proteins to the photoreceptor outer segment membrane.

Molecular Weight: 46.7 kDa

NCBI Accession: NP_000171

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling, Phototransduction