P4HB Protein (full length) (rho-1D4 tag)

Catalog No. ABIN7126029

Product Details

Target: P4HB (Prolyl 4-Hydroxylase, beta Polypeptide (P4HB))

Protein Type: Recombinant

Protein Characteristics: full length

Origin: CHO cells

Source: Insect Cells

Purification tag / Conjugate: This P4HB protein is labelled with rho-1D4 tag.

Application: SDS-PAGE (SDS), Western Blotting (WB), ELISA, Functional Studies (Func), Crystallization (Crys)

Sequence: MRFSCLALLP GVALLLASAR LAAASDVLEL TDENFESRVS DTGSAGLMLV EFFAPWCGHC KRLAPEYEAA ATRLKGIVPL TKVDCTANTN TCNKYGVSGY PTLKIFRDGE EAGAYDGPRT ADGIVSHLKK QAGPASVLLR SEEEFKKFIS DKDASVVGFF RDLFSDGHSE FLKAASNLRD NYRFAHTNVE SLVKEYDDNG EGITLFRPSH LANKFEDKTV VYTEQKMTSG KIKRFIQESI FGICPHMTED NKDLIQSKDL LTAYYDVDYE KNAKGSNYWR NRVMMVAKKF LDAGHKLNFA VASRKTFSHE LSDFGLESTT GEVPVVAIRT AKGEKFVMQE EFSRDGKALE RFLQDYFDGN LKRYLKSEPI PETNDGPVKV VVAENFDDIV NNEDKDVLIE FYAPWCGHCK NLEPKYKELG EKLSKDPNIV IAKMDATAND VPSPYEVKGF PTIYFSPANK KLNPKKYEGG RELNDFINYL QREATTPPII QEEKPKKKKK AQEDL
Sequence without tag. The location of the tag depends on protein. You may also submit your preference when ordering.

Characteristics:

• Made in Germany - from design to production - by highly experienced protein experts.
• CHO Protein disulfide-isomerase Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a custom-made protein and will be made for the first time for your order. This protein will be produced on the basis of on a Custom Service Project. We will make sure that every step in the production is successful from the design of the expression plasmid to the expression and purification of the final protein. Our experts in the lab will ensure that you receive a correctly folded protein.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer. The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Three step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
2. The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
3. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterility: 0.22 μm filtered

Endotoxin Level: Endotoxins have not been removed. Please contact us if you require an endotoxin-free version of this product.

Grade: Crystallography grade

Biological Activity Comment: Protein has not been tested for activity yet.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 150 mM NaCL, 20 mM NaH2PO4 pH 7.4, 10 % glycerol. Note: Isoelectric point of protein taken into account regarding pH .

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: P4HB (Prolyl 4-Hydroxylase, beta Polypeptide (P4HB))

Alternative Name: Protein disulfide-isomerase (P4HB Products)

Synonyms: DSI Protein, ERBA2L Protein, GIT Protein, P4Hbeta Protein, PDI Protein, PDIA1 Protein, PHDB Protein, PO4DB Protein, PO4HB Protein, PROHB Protein, PDA2 Protein, PDIP Protein, PDIR Protein, ERp59 Protein, Pdia1 Protein, Thbp Protein, p55 Protein, XPDIp Protein, pdi Protein, 1810041F13Rik Protein, AI661267 Protein, Pdip Protein, Pdipl Protein, p58 Protein, prolyl 4-hydroxylase subunit beta Protein, protein disulfide isomerase family A member 2 Protein, prolyl 4-hydroxylase, beta polypeptide Protein, protein disulfide isomerase family A member 2 L homeolog Protein, protein disulfide isomerase associated 2 Protein, prolyl 4-hydroxylase subunit beta L homeolog Protein, protein disulfide-isomerase Protein, P4HB Protein, PDIA2 Protein, P4hb Protein, pdia2.L Protein, Pdia2 Protein, p4hb.L Protein, LOC8281065 Protein

UniProt: Q91Z81

Pathways: Maintenance of Protein Location, Cell RedoxHomeostasis, Lipid Metabolism