Histone H2A Protein (full length) (rho-1D4 tag)

Catalog No. ABIN7126067

Product Details

Target: Histone H2A

Protein Type: Recombinant

Protein Characteristics: full length

Origin: CHO cells

Source: Insect Cells

Purification tag / Conjugate: This Histone H2A protein is labelled with rho-1D4 tag.

Application: ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS), Functional Studies (Func)

Sequence: MLISLLLWLF RFRLNSSCLL ILTMSGRGKQ GGKARAKAKT RSSRAGLQFP VGRVHRLLRK GNYSERVGAG APVYLAAVLE YLTAEILELA GNAARDNKKT RIIPRHLQLA IRNDEELNKL LGRVTIAQGG VLPNIQAKPH RYRPGTVALR EIRRYQKSTE LLIRKLPFQR LVREIAQDFK TDLRFQSSAV MALQEACEAY LVGLFEDTNL CAIHAKRVTI MPKDIQLARR IRGERA
Sequence without tag. The location of the tag depends on protein. You may also submit your preference when ordering.

Characteristics:

• Made in Germany - from design to production - by highly experienced protein experts.
• CHO Histone H2A Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a custom-made protein and will be made for the first time for your order. This protein will be produced on the basis of on a Custom Service Project. We will make sure that every step in the production is successful from the design of the expression plasmid to the expression and purification of the final protein. Our experts in the lab will ensure that you receive a correctly folded protein.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer. The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Three step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
2. The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
3. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterility: 0.22 μm filtered

Endotoxin Level: Endotoxins have not been removed. Please contact us if you require an endotoxin-free version of this product.

Grade: Crystallography grade

Biological Activity Comment: Protein has not been tested for activity yet.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 150 mM NaCL, 20 mM NaH2PO4 pH 7.4, 10 % glycerol. Note: Isoelectric point of protein taken into account regarding pH .

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: Histone H2A

Abstract: Histone H2A Products

Synonyms: 5499 Protein, CG5499 Protein, Dmel\\CG5499 Protein, H2A Protein, H2A.F/Z Protein, H2A.X Protein, H2A.Z Protein, H2AV Protein, H2AV_DROM Protein, H2AX Protein, H2AZ Protein, H2Av Protein, H2AvD Protein, H2a.V Protein, H2av Protein, H2avD Protein, HIS Protein, HIS2AV Protein, HIS2AVD Protein, His Protein, His2 Protein, His2AV Protein, His2AvD Protein, HisH2Av Protein, Hist Protein, Hist2av Protein, gamma-H2Av Protein, gamma-HIS2AV Protein, gamma-His2Av Protein, gammaH2Av Protein, h2AvD Protein, his Protein, l(3)05146 Protein, l(3)810 Protein, l(3)97Dd Protein, l(3)L1602 Protein, DDBDRAFT_0205974 Protein, DDBDRAFT_0216271 Protein, DDB_0205974 Protein, DDB_0216271 Protein, RGD1564767 Protein, H2a-221 Protein, H2A-III Protein, HIST1H2AA Protein, Histone H2A variant Protein, putative histone H2A Protein, histone H2A Protein, histone cluster 1 H2A family member F Protein, histone H2A-like Protein, histone cluster 1, H2af Protein, histone2A1 Protein, histone cluster 1, H2A, III Protein, histone cluster 1, H2ac Protein, His2Av Protein, LMJF_17_0280 Protein, H2AX Protein, Hist1h2af Protein, LOC100425378 Protein, his2a1 Protein, HIST1H2A3 Protein, HIST1H2AC Protein, h2a-A Protein

UniProt: G3HDS3