RPSA/Laminin Receptor Protein (full length) (rho-1D4 tag)

Catalog No. ABIN7126076

Product Details

Target: RPSA/Laminin Receptor (RPSA) (Ribosomal Protein SA (RPSA))

Protein Type: Recombinant

Protein Characteristics: full length

Origin: CHO cells

Source: Insect Cells

Purification tag / Conjugate: This RPSA/Laminin Receptor protein is labelled with rho-1D4 tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS), Crystallization (Crys), Functional Studies (Func)

Sequence: MKEEDVLKFL AAGTHLGGTN LDFQMEQYIY KRKSDGIYII NLKRTWEKLL LAARAIVAIE NPADVSVISS RNTGQRAVLK FAAATGATPI AGRFTPGTFT NQIQAAFREP RLLVVTDPRA DHQPLTEASY VNLPTIALCN TDSPLRYVDI AIPCNNKVRV LVESSGKPLI SVHIVRAWS
Sequence without tag. The location of the tag depends on protein. You may also submit your preference when ordering.

Characteristics:

• Made in Germany - from design to production - by highly experienced protein experts.
• CHO 40S ribosomal protein SA Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a custom-made protein and will be made for the first time for your order. This protein will be produced on the basis of on a Custom Service Project. We will make sure that every step in the production is successful from the design of the expression plasmid to the expression and purification of the final protein. Our experts in the lab will ensure that you receive a correctly folded protein.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer. The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Three step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
2. The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
3. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterility: 0.22 μm filtered

Endotoxin Level: Endotoxins have not been removed. Please contact us if you require an endotoxin-free version of this product.

Grade: Crystallography grade

Biological Activity Comment: Protein has not been tested for activity yet.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 150 mM NaCL, 20 mM NaH2PO4 pH 7.4, 10 % glycerol. Note: Isoelectric point of protein taken into account regarding pH .

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: RPSA/Laminin Receptor (RPSA) (Ribosomal Protein SA (RPSA))

Alternative Name: 40S ribosomal protein SA (RPSA Products)

Synonyms: 37LRP Protein, 67LR Protein, LBP/p40 Protein, LRP/LR Protein, LamR Protein, rpsa Protein, LamR1 Protein, RPSA Protein, LAMBR Protein, LAMR1 Protein, LBP Protein, LRP Protein, NEM/1CHD4 Protein, SA Protein, lamR Protein, p40 Protein, 67kDa Protein, 67lr Protein, AL022858 Protein, Lamr Protein, Lamr1 Protein, Lamrl1 Protein, MLR Protein, P40 Protein, P40-3 Protein, P40-8 Protein, lamr1 Protein, zgc:55831 Protein, zgc:77824 Protein, DMRT1 Protein, 40S ribosomal protein SA Protein, 67kD laminin receptor Protein, ribosomal protein SA Protein, rssa Protein, rpsa Protein, RPSA Protein, Rpsa Protein, LOC100144340 Protein

UniProt: G3HQX0

Pathways: Ribonucleoprotein Complex Subunit Organization, Ribosome Assembly