PAPSS1 Protein (His tag)

Catalog No. ABIN7318864

Product Details

Target: PAPSS1 (3'-phosphoadenosine 5'-phosphosulfate Synthase 1 (PAPSS1))

Protein Type: Recombinant

Origin: Human

Source: Human Cells

Purification tag / Conjugate: This PAPSS1 protein is labelled with His tag.

Purpose: Recombinant Human PAPS Synthase 1/PAPSS1 Protein (His Tag)

Sequence: Met 1-Ala624

Characteristics: Recombinant Human Bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 1 is produced by our Mammalian expression system and the target gene encoding Met1-Ala624(O43252) is expressed with a 6His tag at the C-terminus.

Purity: > 95 % as determined by reducing SDS-PAGE.

Endotoxin Level: < 1.0 EU per μg as determined by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Frozen, Liquid

Buffer: Supplied as a 0.2 μm filtered solution of 20 mM Tris,100 mM NaCl,20 % glycerol, pH 8.0.

Storage: -20 °C

Storage Comment: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Target Details

Target: PAPSS1 (3'-phosphoadenosine 5'-phosphosulfate Synthase 1 (PAPSS1))

Alternative Name: PAPS Synthase 1/PAPSS1 (PAPSS1 Products)

Synonyms: ATPSK1 Protein, PAPSS Protein, SK1 Protein, AI325286 Protein, Asapk Protein, papss1 Protein, MGC82588 Protein, atpsk1 Protein, papss Protein, sk1 Protein, PAPSS1 Protein, wu:fc43e12 Protein, zgc:194984 Protein, zgc:194985 Protein, CG8363 Protein, DmPAPSS Protein, Dmel\\CG8363 Protein, PAPS Protein, Paps Protein, l(3)76BDn Protein, paps Protein, 3'-phosphoadenosine 5'-phosphosulfate synthase 1 Protein, bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 1 Protein, bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 2 Protein, PAPS synthetase Protein, PAPSS1 Protein, Papss1 Protein, papss1 Protein, papss1.L Protein, LOC100543301 Protein, LOC100637112 Protein, Papss Protein

Background:

Background: PAPSS1 is a bifunctional enzyme with both ATP sulfurylase and APS kinase activity. In the N-terminal section, it belongs to the APS kinase family, while the C-terminal section belongs to the sulfate adenylyltransferase family. PAPSS1 can be inhibited by chlorate, and is expressed in many tissues, such as high endothelial venules (HEV) cells and in cartilage. PAPSS1 mediates two steps in the sulfate activation pathway. The first step is the transfer of a sulfate group to ATP to yield adenosine 5'-phosphosulfate (APS), and the second step is the transfer of a phosphate group from ATP to APS yielding 3'-phosphoadenylylsulfate. In mammals, PAPS is the sole source of sulfate, APS appears to be only an intermediate in the sulfate-activation pathway. PAPSS1 also involved in the biosynthesis of sulfated L-selectin ligands in endothelial cells.

Synonym: Bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 1,PAPS synthase 1,PAPSS 1,Sulfurylase kinase 1,SK 1,ATPSK1, PAPSS

Molecular Weight: 71.9 kDa

UniProt: O43252

Pathways: Glycosaminoglycan Metabolic Process, Ribonucleoside Biosynthetic Process