ACOX1 Protein (His tag)
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- Target See all ACOX1 Proteins
- ACOX1 (Acyl-CoA Oxidase 1, Palmitoyl (ACOX1))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- Baculovirus infected Insect Cells
- Purification tag / Conjugate
- This ACOX1 protein is labelled with His tag.
- Purpose
- Recombinant Human ACOX1/aox Protein (His Tag)
- Sequence
- Met 1-Leu 660
- Characteristics
- A DNA sequence encoding the human ACOX1 (AAH08767.1) (Met 1-Leu 660) was expressed, with a polyhistidine tag at the N-terminus.
- Purity
- > 85 % as determined by reducing SDS-PAGE.
- Endotoxin Level
- < 1.0 EU per μg as determined by the LAL method.
- Top Product
- Discover our top product ACOX1 Protein
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Acyl-CoA Oxidase 1, Palmitoyl (ACOX1) (AA 306-395) protein (His-IF2DI Tag)
ACOX1 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Acyl-CoA Oxidase 1, Palmitoyl (ACOX1) (AA 1-660) protein (GST tag)ACOX1 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Acyl-CoA Oxidase 1, Palmitoyl (ACOX1) (AA 1-660) protein (Strep Tag)Crystallography grade ACOX1 Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
Acyl-CoA Oxidase 1, Palmitoyl (ACOX1) (AA 1-661) protein (Strep Tag)Crystallography grade ACOX1 Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Please refer to the printed manual for detailed information.
- Buffer
- Lyophilized from sterile 20 mM Tris, 500 mM NaCl, pH 7.0, 20 % glycerol, 3 mM DTT
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- Target
- ACOX1 (Acyl-CoA Oxidase 1, Palmitoyl (ACOX1))
- Alternative Name
- ACOX1/aox (ACOX1 Products)
- Synonyms
- zgc:92584 Protein, wu:fb59h12 Protein, zgc:114033 Protein, aco Protein, acox Protein, ACOX Protein, PALMCOX Protein, SCOX Protein, RATACOA1 Protein, AOX Protein, Acox Protein, D130055E20Rik Protein, Paox Protein, PCOX1 Protein, acyl-CoA oxidase 1 Protein, acyl-CoA oxidase 1, palmitoyl Protein, acyl-CoA oxidase 1, palmitoyl L homeolog Protein, acyl-Coenzyme A oxidase 1, palmitoyl Protein, ACOX1 Protein, acox1 Protein, acox1.L Protein, Acox1 Protein
- Background
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Background: Peroxisomal acyl-coenzyme A oxidase 1(ACOX1 or AOX) is the first enzyme of the fatty acid beta-oxidation pathway and belongs to the Acyl-CoA oxidase family. Human liver peroxisomes contain two acyl-CoA oxidases, namely, palmitoyl-CoA oxidase (ACOX1/AOX) and a branched chain acyl-CoA oxidase. The palmitoyl-CoA oxidase (ACOX1/AOX) oxidizes the CoA esters of straight chain fatty acids and prostaglandins and donates electrons directly to molecular oxygen, thereby producing H2O2. Human ACOX1/AOX is a protein of 661-amino acids, including the carboxyl-terminal sequence(Ser-Lys-Leu) known as a minimal peroxisome-targeting signal. Human ACOX1/AOX, the first and rate-limiting enzyme of the peroxisomal β-oxidation pathway, has two isoforms including ACOX1a and ACOX1b, transcribed from a single gene. The human ACOX1b isoform is more effective than the ACOX1a isoform in reversing the Acox1 null phenotype in the mouse partly because of the Substrate utilization differences.
Synonym: ACOX;PALMCOX;SCOX
- Molecular Weight
- 76.7 kDa
- Pathways
- Regulation of Lipid Metabolism by PPARalpha, Monocarboxylic Acid Catabolic Process
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