ALOX5AP Protein (His tag)

Catalog No. ABIN7194300

Product Details

Target: ALOX5AP (Arachidonate 5-Lipoxygenase-Activating Protein (ALOX5AP))

Protein Type: Recombinant

Origin: Human

Source: Baculovirus infected Insect Cells

Purification tag / Conjugate: This ALOX5AP protein is labelled with His tag.

Purpose: Recombinant Human ALOX5AP/FLAP Protein (His Tag)

Sequence: Met 1-Pro 161

Characteristics: A DNA sequence encoding the full length of human ALOX5AP (NP_001620.2) (Met 1-Pro 161) was fused with a polyhistidine tag at the carboxy-terminus.

Purity: > 90 % as determined by reducing SDS-PAGE.

Endotoxin Level: < 1.0 EU per μg as determined by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile 20 mM Tris, 150 mM NaCl, 10 % glycerol, pH 7.4, 0.5 % triton, 5 mM DTT

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: ALOX5AP (Arachidonate 5-Lipoxygenase-Activating Protein (ALOX5AP))

Alternative Name: ALOX5AP/FLAP (ALOX5AP Products)

Synonyms: fj65d02 Protein, zgc:63982 Protein, wu:fj65d02 Protein, flap Protein, al5ap Protein, Alox5ap Protein, ALOX5AP Protein, Flap Protein, FLAP Protein, arachidonate 5-lipoxygenase-activating protein Protein, arachidonate 5-lipoxygenase activating protein Protein, alox5ap Protein, ALOX5AP Protein, Alox5ap Protein

Background:

Background: Arachidonate 5-Lipoxygenase-Activating Protein (ALOX5AP), also known as FLAP, belongs to the MAPEG family. ALOX5AP/FLAP is an essential partner of 5-LO for this process. The FLAP (ALOX5AP) gene has been linked to risk for myocardial infarction, stroke and restenosis, reigniting pharmaceutical interest in this target. It had been found that ALOX5AP/FLAP is a key enzyme in leukotriene formation, in both human pulmonary microvascular endothelial cells and a transformed human brain endothelial cell line. In addition, the protein FLAP has recently been identified as an emerging target in metabolic disease. In fact, FLAP is overexpressed in the adipose tissue of patients and experimental animals with obesity.

Synonym: FLAP

Molecular Weight: 19 kDa

NCBI Accession: NP_001620