ACLY Protein (His tag)

Catalog No. ABIN7194322

Product Details

Target: ACLY (ATP Citrate Lyase (ACLY))

Protein Type: Recombinant

Origin: Human

Source: Baculovirus infected Insect Cells

Purification tag / Conjugate: This ACLY protein is labelled with His tag.

Purpose: Recombinant Human ACLY/acly/ATP citrate lyase Protein (His Tag)

Sequence: Met 1-Met 1101

Characteristics: A DNA sequence encoding the human ACLY (P53396) (Met 1-Met 1101) was expressed, with a polyhistidine tag at the N-terminus.

Purity: > 90 % as determined by reducing SDS-PAGE.

Endotoxin Level: < 1.0 EU per μg as determined by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile 20 mM Tris, 500 mM NaCl, pH 8.0, 10 % glycerol

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: ACLY (ATP Citrate Lyase (ACLY))

Alternative Name: ACLY/acly/ATP citrate lyase (ACLY Products)

Synonyms: ACLY Protein, DDBDRAFT_0205389 Protein, DDBDRAFT_0235360 Protein, DDB_0205389 Protein, DDB_0235360 Protein, ACL Protein, BcDNA:LD21334 Protein, CG8322 Protein, DmATPCL Protein, Dmel\\CG8322 Protein, anon-WO0140519.179 Protein, dATPCL Protein, l(2)01466 Protein, l(2)k09217 Protein, n(2)k09217 Protein, ATPCL Protein, CLATP Protein, A730098H14Rik Protein, AW538652 Protein, Clatp Protein, acly Protein, cb722 Protein, zgc:92008 Protein, ATP citrate lyase Protein, ATP citrate lyase S homeolog Protein, ATP-citrate synthase Protein, citrate synthase, mitochondrial Protein, atp-citrate synthase Protein, ATP citrate lyase a Protein, ACLY Protein, acly.S Protein, LOC587157 Protein, acly Protein, ATPCL Protein, LOC5564509 Protein, CpipJ_CPIJ010291 Protein, Bm1_26245 Protein, ACL Protein, Acly Protein, aclya Protein

Background:

Background: ATP citrate lyase, also known as Acly or Acl, is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is composed of two polymer chains which are polypeptides in human. ATP citrate lyase is responsible for catalyzing the conversion of citrate and CoA into acetyl-CoA and oxaloacetate, along with the hydrolysis of ATP. A definitive role for ATP citrate lyase in tumorigenesis has emerged from ATP citrate lyase RNAi and chemical inhibitor studies, showing that ATP citrate lyase inhibition limits tumor cell proliferation and survival and induces differentiation in vitro. In vivo, it reduces tumor growth leading to a cytostatic effect and induces differentiation. 

Synonym: ACL;ATPCL;CLATP

Molecular Weight: 123 kDa

UniProt: P53396

Pathways: Warburg Effect