BLMH Protein (His tag)

Catalog No. ABIN7194397

Product Details

Target: BLMH (Bleomycin Hydrolase (BLMH))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This BLMH protein is labelled with His tag.

Purpose: Recombinant Mouse BLMH/BLM Hydrolase Protein (His Tag)(Active)

Sequence: Asn 2-Glu 455

Characteristics: A DNA sequence encoding the mouse BLMH (NP_848760.1) (Asn 2-Glu 455) was expressed, with a polyhistide tag at the N-terminus.

Purity: > 95 % as determined by SDS-PAGE

Biological Activity Comment: Measured by its ability to hydrolyze Met-AMC. The specific activity is >500 pmoles/min/μg.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile 50 mM Tris, 0.15M NaCl, 10 % glycerol, pH 8.0

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: BLMH (Bleomycin Hydrolase (BLMH))

Alternative Name: BLMH/BLM Hydrolase (BLMH Products)

Synonyms: AI035728 Protein, Bh Protein, Bmh Protein, wu:fb13c01 Protein, zgc:66261 Protein, bh Protein, bmh Protein, BH Protein, BMH Protein, bleomycin hydrolase Protein, Bleomycin hydrolase Protein, bleomycin hydrolase S homeolog Protein, BLMH Protein, pepC Protein, LOC5569945 Protein, Apre_0140 Protein, Apar_0899 Protein, Smon_1289 Protein, Kfla_1242 Protein, Snas_3912 Protein, BLJ_1941 Protein, Olsu_1085 Protein, PGTDC60_0106 Protein, Blmh Protein, blmh Protein, blmh.S Protein

Background:

Background: The papain superfamily member bleomycin hydrolase (BLMH) is a cytoplasmic cysteine peptidase that is highly conserved through evolution. The only known activity of the enzyme is metabolic inactivation of the glycopeptide bleomycin (BLM), an essential component of combination chemotherapy regimens for cancer. The papain superfamily member bleomycin hydrolase (BLMH) is a neutral cysteine protease with structural similarity to a 20S proteasome. Bleomycin (BLM), a clinically used glycopeptide anticancer agent. BLMH is an essential protectant against BLM-induced death and has an important role in neonatal survival and in maintaining epidermal integrity. Sequencing revealed several putative sites phosphorylated by different types of protein kinases, but no signal sequence, transmembrane domain, N-linked glycosylation site or DNA-binding motif.

Synonym: AI035728,Bh,Bmh

Molecular Weight: 53.3 kDa

NCBI Accession: NP_848760