BDNF Protein (AA 129-247) (His tag)

Catalog No. ABIN7319738

This Recombinant BDNF protein is produced in Escherichia coli (E. coli).

Quick Overview for BDNF Protein (AA 129-247) (His tag) (ABIN7319738)

Target: BDNF (Brain-Derived Neurotrophic Factor (BDNF))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human, Rat, Mouse

Source: Escherichia coli (E. coli)

Application: Cell Culture (CC)

Purity: > 98 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 129-247

Purification tag / Conjugate: This BDNF protein is labelled with His tag.

Purpose: Recombinant Human/Mouse/Rat BDNF Protein

Sequence: His129-Arg247

Characteristics: Recombinant Human/Mouse/Rat BDNF is produced by our E.coli expression system and the target gene encoding His129-Arg247 is expressed.

Sterility: 0.2 μm filtered

Endotoxin Level: < 0.1 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Measure by its ability to induce proliferation in BaF3 cells transfected with TrkB. The ED₅₀ for this effect is <2 ng/mL.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from sterile 20 mM sodium citrate, 0.2 M NaCl, pH 3.5.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: BDNF (Brain-Derived Neurotrophic Factor (BDNF))

Alternative Name: BDNF

Background: Abrineurin,BDNF,Brain-Derived Neurotrophic Factor,Brain-Derived Neurotrophic Factor (BDNF) is a member of the neurotrophin family. Along with other structurally related neurotrophic factors NGF, NT-3 and NT-4, BDNF binds with high affinity to the TrkB kinase receptor. It also binds with the LNGFR (for low-affinity nerve growth factor receptor, also known as p75). BDNF promotes the survival, growth and differentiation of neurons. It serves as a major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. BDNF expression is altered in neurodegenerative disorders such as Parkinson's and Alzheimer's disease.

Molecular Weight:

Calculated MW: 14.5 kDa

Observed MW: 14 kDa

Gene ID: 627

UniProt: P23560

Pathways: RTK Signaling, Synaptic Membrane, Feeding Behaviour, Dicarboxylic Acid Transport, Regulation of long-term Neuronal Synaptic Plasticity