CA4 Protein (His tag)

Catalog No. ABIN7194567

Product Details

Target: CA4 (Carbonic Anhydrase IV (CA4))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: HEK-293 Cells

Purification tag / Conjugate: This CA4 protein is labelled with His tag.

Purpose: Recombinant Mouse Carbonic Anhydrase 4/CA4 Protein (His Tag)(Active)

Sequence: Met 1-Ser 277

Characteristics: A DNA sequence encoding the mouse Car4 (NP_031633.1) without the C-terminal propeptide (Met 1-Ser 277) was expressed,with a C-terminal polyhistidine tag.

Purity: > 98 % as determined by SDS-PAGE

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Measured by its esterase activity. The specific activity is >10 pmoles/min/μg.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, pH 7.4

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: CA4 (Carbonic Anhydrase IV (CA4))

Alternative Name: Carbonic Anhydrase 4/CA4 (CA4 Products)

Synonyms: CAIV Protein, Car4 Protein, RP17 Protein, AW456718 Protein, Ca4 Protein, ca4 Protein, caiv Protein, car4 Protein, rp17 Protein, CA4 Protein, zgc:171842 Protein, carbonic anhydrase 4 Protein, carbonic anhydrase 4 S homeolog Protein, carbonic anhydrase IV a Protein, CA4 Protein, Car4 Protein, ca4 Protein, ca4.S Protein, Ca4 Protein, ca4a Protein

Background:

Background: The carbonic anhydrases (or carbonate dehydratases) are classified as metalloenzyme for its zinc ion prosthetic group and form a family of enzymes that catalyze the rapid interconversion of carbon dioxide and water to bicarbonate and protons, a reversible reaction that takes part in maintaining acid-base balance in blood and other tissues. The carbonic anhydrasekl (CA) family consists of at least 11 enzymatically active members and a few inactive homologous proteins. Carbonic anhydrase IV (CAIV) is a membrane-associated enzyme anchored to plasma membrane surfaces by a phosphatidylinositol glycan linkage. CAIV is a high-activity isozyme in CO2 hydration comparable to that of CAII. Furthermore, CAIV is more active in HCO3- dehydration than is CAII. However, the esterase activity of CAIV is decreased 150-fold compared to CAII.

Synonym: CA4,CAIV,CA-IV,Car4,Carbonate dehydratase IV,carbonic anhydrase 4,carbonic anhydrase IVRP17,carbonic dehydratase IV,EC4.2.1.1,retinitis pigmentosa 17,RP17

Molecular Weight: 31 kDa

NCBI Accession: NP_031633