CSNK2A2 Protein (GST tag,His tag)

Catalog No. ABIN7317080

Product Details

Target: CSNK2A2 (Casein Kinase 2, alpha Prime Polypeptide (CSNK2A2))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Baculovirus infected Insect Cells

Purification tag / Conjugate: This CSNK2A2 protein is labelled with GST tag,His tag.

Purpose: Recombinant Human CSNK2A2/CK2A2 Protein (His & GST Tag)(Active)

Sequence: Met 1-Arg 350

Characteristics: A DNA sequence encoding the human CSNK2A2 (NP_001887.1) (Met 1-Arg 350) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Purity: > 92 % as determined by reducing SDS-PAGE.

Endotoxin Level: < 1.0 EU per μg as determined by the LAL method.

Biological Activity Comment: The specific activity was determined to be 30 nmol/min/mg using casein as substrate.

Application Details

Restrictions: For Research Use only

Handling

Format: Frozen, Liquid

Buffer: Supplied as sterile 50 mM Tris, 500 mM NaCl, 0.5 mM PMSF, 0.5 mM TCEP, 10 % glycerol, pH 8.5

Storage: -20 °C

Storage Comment: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Target Details

Target: CSNK2A2 (Casein Kinase 2, alpha Prime Polypeptide (CSNK2A2))

Alternative Name: CSNK2A2/CK2A2 (CSNK2A2 Products)

Synonyms: CK2A2 Protein, CSNK2A1 Protein, 1110035J23Rik Protein, C77789 Protein, CK2 Protein, CSNK2A2 Protein, ck2a2 Protein, csnk2a1 Protein, wu:fi09h12 Protein, casein kinase 2 alpha 2 Protein, casein kinase 2, alpha prime polypeptide Protein, casein kinase 2 alpha 2 L homeolog Protein, casein kinase 2, alpha prime polypeptide a Protein, CSNK2A2 Protein, Csnk2a2 Protein, csnk2a2.L Protein, csnk2a2 Protein, csnk2a2a Protein

Background:

Background: Casein kinase II subunit alpha', also known as CSNK2A2 and CK2A2, is a member of the protein kinase superfamily, Ser/Thr protein kinase family and CK2 subfamily. Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. The alpha and alpha' chains contain the catalytic site. CSNK2A2 is a tetramer composed of an alpha chain, an alpha' and two beta chains. It is also component of a CK2-SPT16-SSRP1 complex composed of SSRP1, SUPT16H, CSNK2A1, CSNK2A2 and CSNK2B, the complex associating following UV irradiation. Protein kinase casein kinase II (Ck2) is a cyclic-AMP and calcium-independent serine-threonine kinase that is composed of two catalytic subunits (alpha and alpha') and two regulatory beta-subunits. Ck2 is not a casein kinase in vivo, but over 100 substrates are known. The highly conserved amino acid sequences of its subunits and their broad expression suggest that Ck2 may have a fundamental role in cell function. Ck2 has been implicated in DNA replication, regulation of basal and inducible transcription, translation and control of metabolism. The Ck2alpha and Ck2alpha' isoforms (products of the genes Csnk2a1 and Csnk2a2, respectively) are highly homologous, the reason for their redundancy and evolutionary conservation is unknown. CSNK2A2 may be a candidate gene for these inherited syndromes.

Synonym: CK2A2,CSNK2A1

Molecular Weight: 69 kDa

NCBI Accession: NP_001887

Pathways: SARS-CoV-2 Protein Interactome