CHEK2 Protein (His-GST)

Catalog No. ABIN7320011

This Recombinant CHEK2 protein is produced in Baculovirus infected Insect Cells.

Quick Overview for CHEK2 Protein (His-GST) (ABIN7320011)

Target: CHEK2 (Checkpoint Kinase 2 (CHEK2))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: Baculovirus infected Insect Cells

Purity: > 90 % as determined by reducing SDS-PAGE.

Product Details

Purification tag / Conjugate: This CHEK2 protein is labelled with His-GST.

Purpose: Recombinant Mouse CHK2/CHEK2 Protein (His & GST Tag)

Sequence: Mey 1-Leu 546

Characteristics: A DNA sequence encoding the mouse CHEK2 (Q9Z265) (Mey 1-Leu 546) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Sterility: 0.2 μm filtered

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Kinase activity untested

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Supplied as sterile solution of 20 mM Tris, 500 mM NaCl, pH 8.0

Storage: -20 °C

Storage Comment: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Expiry Date: 6 months

Target Details

Target: CHEK2 (Checkpoint Kinase 2 (CHEK2))

Alternative Name: CHK2/CHEK2

Background: CHK2,Cds1,HUCDS1,Rad53,In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by CHEK2 gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded CHEK2 protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in CHEK2s gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases. Several transcript variants encoding different isoforms have been found for this gene.

Molecular Weight:

Calculated MW: 89.0 kDa

Observed MW: 90 kDa

UniProt: Q9Z265

Pathways: p53 Signaling, Apoptosis, Cell Division Cycle