DAP Kinase 1 Protein (AA 1-363) (GST tag,His tag)
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- Target See all DAP Kinase 1 (DAPK1) Proteins
- DAP Kinase 1 (DAPK1) (Death-Associated Protein Kinase 1 (DAPK1))
- Protein Type
- Recombinant
- Biological Activity
- Active
- Protein Characteristics
- AA 1-363
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Origin
- Human
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Source
- Baculovirus infected Insect Cells
- Purification tag / Conjugate
- This DAP Kinase 1 protein is labelled with GST tag,His tag.
- Purpose
- Recombinant Human DAPK1/DAP Kinase 1 Protein (aa 1-363, His & GST Tag)(Active)
- Sequence
- Met 1-Leu 363
- Characteristics
- A DNA sequence encoding the N-terminal segment of human DAPK1 (P53355-1) (Met 1-Leu 363) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
- Purity
- > 80 % as determined by reducing SDS-PAGE.
- Endotoxin Level
- < 1.0 EU per μg as determined by the LAL method.
- Biological Activity Comment
- The specific activity was determined to be 20 nmol/min/mg using synthetic R11-S6-Peptide (R11-IAKRRRLSSLRASTSKSESSQK) as substrate.
- Top Product
- Discover our top product DAPK1 Protein
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Death-Associated Protein Kinase 1 (DAPK1) (AA 1-289) (Active) protein (His tag)
DAPK1 Origin: Human Host: Insect Cells Recombinant SDS, Func Active
Death-Associated Protein Kinase 1 (DAPK1) (AA 1-363) (Active) protein (His-GST)DAPK1 Origin: Human Host: Insect cells (Sf9) Recombinant SDS, Func Active
Death-Associated Protein Kinase 1 (DAPK1) (AA 1-368) protein (GST tag)DAPK1 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Death-Associated Protein Kinase 1 (DAPK1) (AA 1211-1310) protein (GST tag)DAPK1 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Death-Associated Protein Kinase 1 (DAPK1) protein (Myc-DYKDDDDK Tag)DAPK1 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Restrictions
- For Research Use only
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- Format
- Frozen, Liquid
- Buffer
- Supplied as sterile 20 mM Tris, 500 mM NaCl, pH 8.0, 10 % glycerol
- Storage
- -20 °C
- Storage Comment
- Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
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- Target
- DAP Kinase 1 (DAPK1) (Death-Associated Protein Kinase 1 (DAPK1))
- Alternative Name
- DAPK1/DAP Kinase 1 (DAPK1 Products)
- Synonyms
- DAPK1 Protein, MGC81366 Protein, si:ch211-66i11.1 Protein, wu:fj09c03 Protein, dapk Protein, MGC83745 Protein, 2310039H24Rik Protein, 2810425C21Rik Protein, AI642003 Protein, D13Ucla1 Protein, DAP-Kinase Protein, DAPK Protein, death-associated protein kinase 1 Protein, death associated protein kinase 1 L homeolog Protein, death associated protein kinase 1 Protein, death associated protein kinase 1 S homeolog Protein, LOC427465 Protein, dapk1.L Protein, DAPK1 Protein, dapk1 Protein, dapk1.S Protein, Dapk1 Protein
- Background
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Background: Death-associated protein kinase 1, also known as DAP kinase 1, DAPK1 and DAPK, is a cytoplasm protein which belongs to the protein kinase superfamily, CAMK Ser / Thr protein kinase family and DAP kinase subfamily. DAPK1 contains ten ANK repeats, one death domain and one protein kinase domain. DAPK1 is a calcium / calmodulin-dependent serine/threonine kinase which acts as a positive regulator of apoptosis. DAPK1 gene is a candidate tumor suppressor (TSG) and the abnormal methylation of DAPK1 gene has been found in many carcinomas. DAPK1 over-expression can induce cell apoptosis and inhibit tumor cell metastasis. DAPK1 gene over-expression could suppress PGCl3 cells malignant phenotype, inhibit PGCl3 cells growth, invasive, migration and adhesion ability, upregulate p53 gene and downregulate bcl-2 gene. Loss of activity of death-associated protein kinase 1 ( DAPK1 ) may be an independent factor affecting survival of non-small cell lung cancer patients. DAPK1 promoter methylation might play a significant role in the progression of chronic myeloid leukemia ( CML ).
Synonym: DAPK
- Molecular Weight
- 69.4 kDa
- Pathways
- MAPK Signaling, Interferon-gamma Pathway
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