DUSP14 Protein (His tag,MBP tag)

Catalog No. ABIN7317376

Product Details

Target: DUSP14 (Dual Specificity Phosphatase 14 (DUSP14))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This DUSP14 protein is labelled with His tag,MBP tag.

Purpose: Recombinant Human DUSP14/MKP-6 Protein (His & MBP Tag)

Sequence: Met 1 -His 191

Characteristics: A DNA sequence encoding the human DUSP14 (O95147) (Met 1 -His 191) was fused with an N-terminal polyhistidine-tagged MBP tag at the N-terminus.

Purity: > 88 % as determined by reducing SDS-PAGE.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, pH 7.5

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: DUSP14 (Dual Specificity Phosphatase 14 (DUSP14))

Alternative Name: DUSP14/MKP-6 (DUSP14 Products)

Synonyms: MGC84083 Protein, zgc:101746 Protein, DUSP14 Protein, mkp-l Protein, mkp6 Protein, MKP-L Protein, MKP6 Protein, 1110014C10Rik Protein, 2310042C07Rik Protein, D11Ertd395e Protein, Mkp6 Protein, dual specificity phosphatase 14 Protein, dual specificity phosphatase 14 L homeolog Protein, DUSP14 Protein, dusp14.L Protein, dusp14 Protein, Dusp14 Protein

Background:

Background: Dual specific phosphatase 14 / MAP-kinase phophatase-6 (DUSP14 / MKP6) is a member of Dual-specificity phosphatases that is a subclass of protein tyrosine phosphatases (PTP) families that can dephosphorylate bothe phosphotyrosine and phosphoserine / phosphothreonine residues in substrates. Unlike many other DUSPs, DUSP14 only contains a catalytic domain within the C-terminal region. In signal transduction, DUSP14 has been considered as negative regulator of the mitogen-activated protein kinase (MAPK) / extracellular signal-regulated kinase 1 / 2 (ERK 1 / 2) pathway. DUSP14 phosphatase activity has been confirmed to be inhibited by PTP inhibitor IV. PTP inhibitor binds to the catalytic site of DUSP14. PTP inhibitor IV effectively and specifically inhibited DUSP14-mediated dephosphorylation of JNK, a member of the mitogen-activated protein kinase (MAPK) family through dephosphorylation of both the Ser / Thr and Tyr residues of MAPKs. 

Synonym: MKP-L;MKP6

Molecular Weight: 65 kDa

UniProt: O95147