EGFR Protein (AA 668-1210) (His-GST)

Catalog No. ABIN7317119

This Recombinant EGFR protein is expressed in Baculovirus infected Insect Cells.

Quick Overview for EGFR Protein (AA 668-1210) (His-GST) (ABIN7317119)

Target: EGFR (Epidermal Growth Factor Receptor (EGFR))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Baculovirus infected Insect Cells

Purity: > 85 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 668-1210

Purification tag / Conjugate: This EGFR protein is labelled with His-GST.

Purpose: Recombinant Human EGFR/ErbB1 Protein (aa 668-1210, His&GST Tag)

Sequence: Met 668-Ala 1210

Characteristics: A DNA sequence encoding the cytoplasmic domain (Met 668-Ala 1210) of human EGFR (NP_005219) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Sterility: 0.2 μm filtered

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: The specific activity was determined to be 105 nmol/min/mg using Poly(Glu:Tyr) 4:1 as substrate.

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Supplied as sterile solution of 20 mM Tris, 500 mM NaCl, 10 % glycerol, pH 7.4

Storage: -20 °C

Storage Comment: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Expiry Date: 6 months

Target Details

Target: EGFR (Epidermal Growth Factor Receptor (EGFR))

Alternative Name: EGFR/ErbB1

Background: ERBB;ERBB1;HER1;NISBD2;PIG61;mENA,As a member of the epidermal growth factor receptor (EGFR) family; EGFR protein is type I transmembrane glycoprotein that binds a subset of EGF family ligands including EGF; amphiregulin; TGF-α; betacellulin; etc. EGFR protein plays a crucial role in signaling pathway in the regulation of cell proliferation; survival and differentiation. Binding of a ligand induces EGFR protein homo- or heterodimerization; the subsequent tyrosine autophosphorylation and initiates various down stream pathways (MAPK; PI3K/PKB and STAT). In addition; EGFR signaling also has been shown to exert action on carcinogenesis and disease progression; and thus EGFR protein is proposed as a target for cancer therapy currently.

Molecular Weight: 89.1 kDa

Gene ID: 1956

NCBI Accession: NP_005219

UniProt: P00533

Pathways: NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Stem Cell Maintenance, Hepatitis C, Positive Regulation of Response to DNA Damage Stimulus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation, S100 Proteins