FCGR1A Protein (His tag)

Catalog No. ABIN7195675

Product Details

Target: FCGR1A (Fc Fragment of IgG, High Affinity Ia, Receptor (CD64) (FCGR1A))

Protein Type: Recombinant

Biological Activity: Active

Origin: Rat

Source: HEK-293 Cells

Purification tag / Conjugate: This FCGR1A protein is labelled with His tag.

Purpose: Recombinant Rat FCGR1A/CD64 Protein (His Tag)(Active)

Sequence: Met 1-Pro 285

Characteristics: A DNA sequence encoding the rat FCGR1A (NP_001094306.1) extracellular domain (Met 1-Pro 285) was expressed, fused with a polyhistidine tag at the C-terminus.

Purity: > 95 % as determined by SDS-PAGE

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method

Biological Activity Comment: Measured by its binding ability in a functional ELISA. Immobilized Rat CD64-his at 10 μg/ml (100 μl/well) can bind biotinylated human IgG1, The EC50 of biotinylated human IgG1 is 0.04-0.08 μg/ml.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, pH 7.4

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: FCGR1A (Fc Fragment of IgG, High Affinity Ia, Receptor (CD64) (FCGR1A))

Alternative Name: FCGR1A/CD64 (FCGR1A Products)

Synonyms: CD64 Protein, CD64A Protein, FCRI Protein, IGFR1 Protein, FcgammaRI Protein, Fcgr1 Protein, Fcgr1b Protein, AI323638 Protein, AV092959 Protein, IGGHAFC Protein, FCGR1A Protein, Fc fragment of IgG receptor Ia Protein, Fc receptor, IgG, high affinity I Protein, FCGR1A Protein, Fcgr1a Protein, Fcgr1 Protein

Background:

Background: High affinity immunoglobulin gamma Fc receptor I, also known as FCGR1 and CD64, is an integral membrane glycoprotein and a member of the immunoglobulin superfamily. CD64 is a high affinity receptor for the Fc region of IgG gamma and functions in both innate and adaptive immune responses. Receptors that recognize the Fc portion of IgG function in the regulation of immune response and are divided into three classes designated CD64, CD32, and CD16. CD64 is structurally composed of a signal peptide that allows its transport to the surface of a cell, three extracellular immunoglobulin domains of the C2-type that it uses to bind antibody, a hydrophobic transmembrane domain, and a short cytoplasmic tail. CD64 is constitutively found on only macrophages and monocytes, but treatment of polymorphonuclear leukocytes with cytokines like IFNγ and G-CSF can induce CD64 expression on these cells. The inactivation of the mouse CD64 resulted in a wide range of defects in antibody Fc-dependent functions. Mouse CD64 is an early participant in Fc-dependent cell activation and in the development of immune responses.

Synonym: FcgammaRI;Fcgr1;Fcgr1b

Molecular Weight: 31.6 kDa

NCBI Accession: NP_001094306

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process