FGFR1 Protein (His-GST)

Catalog No. ABIN7317102

This Recombinant FGFR1 protein is expressed in Baculovirus infected Insect Cells.

Quick Overview for FGFR1 Protein (His-GST) (ABIN7317102)

Target: FGFR1 (Fibroblast Growth Factor Receptor 1 (FGFR1))

Protein Type: Recombinant

Origin: Human

Source: Baculovirus infected Insect Cells

Purity: > 93 % as determined by reducing SDS-PAGE.

Product Details

Purification tag / Conjugate: This FGFR1 protein is labelled with His-GST.

Purpose: Recombinant Human FGFR1/CD331 Protein (His & GST Tag)

Sequence: Gly 311-Arg 731

Characteristics: A DNA sequence encoding the human FGFR1 isoform 4 (NP_075594.1) cytoplasmic domain (Gly 311-Arg 731) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Sterility: 0.2 μm filtered

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Not validated for activity

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Supplied as sterile solution of 50 mM Tris, 100 mM NaCl, pH 8.0, 20 % glycerol, 0.3 mM DTT

Preservative: Dithiothreitol (DTT)

Precaution of Use: This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Expiry Date: 6 months

Target Details

Target: FGFR1 (Fibroblast Growth Factor Receptor 1 (FGFR1))

Alternative Name: FGFR1/CD331

Background: BFGFR,CD331,CEK,FGFBR,FGFR-1,FLG,FLT-2,FLT2,HBGFR,HH2,HRTFDS,KAL2,N-SAM,OGD,bFGF-R-1,FGFR1, also known as CD331, belongs to the fibroblast growth factor receptor subfamily where amino acid sequence is highly conserved between members and throughout evolution. FGFR family members differ from one another in their ligand affinities and tissue distribution. Fibroblast growth factors (FGFs) (FGF1 - 10 and 16 - 23) are mitogenic signaling molecules that have roles in angiogenesis, wound healing, cell migration, neural outgrowth and embryonic development. FGFs bind heparan sulfate glycosaminoglycans, which facilitates dimerization (activation) of FGF receptors. FGFR1 is a full-length representative protein consists of an extracellular region, composed of three immunoglobulin-like domains, a single hydrophobic membrane-spanning segment and a cytoplasmic tyrosine kinase domain. The extracellular portion of FGFR1 interacts with fibroblast growth factors, setting in motion a cascade of downstream signals, ultimately influencing mitogenesis and differentiation. This particular family member binds both acidic and basic fibroblast growth factors and is involved in limb induction. CD331 can be detected in astrocytoma, neuroblastoma and adrenal cortex cell lines. Some isoforms are detected in foreskin fibroblast cell lines, however isoform 17, isoform 18 and isoform 19 are not detected in these cells. Defects in FGFR1 are a cause of Pfeiffer syndrome ,idiopathic hypogonadotropic hypogonadism, Kallmann syndrome type 2, osteoglophonic dysplasia and trigonocephaly non-syndromic.

Molecular Weight:

Calculated MW: 75.0 kDa

Observed MW: 75 kDa

Gene ID: 2260

NCBI Accession: NP_075594

UniProt: P11362

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Sensory Perception of Sound, Stem Cell Maintenance, S100 Proteins