GSK3 beta Protein (His tag)

Catalog No. ABIN7320446

Product Details

Target: GSK3 beta (GSK3b) (Glycogen Synthase Kinase 3 beta (GSK3b))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: Baculovirus infected Insect Cells

Purification tag / Conjugate: This GSK3 beta protein is labelled with His tag.

Purpose: Recombinant Mouse GSK3B Protein (His Tag)(Active)

Sequence: Met 1-Thr 420

Characteristics: A DNA sequence encoding the mouse GSK3B (Q9WV60) (Met 1-Thr 420) was fused with a polyhistidine tag at the N-terminus.

Purity: > 85 % as determined by SDS-PAGE

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: 1. The specific activity was determined to be 61 nmol/min/mg using synthetic Phospho-Glycogen Synthase Peptide-2 (YRRAAVPPSPSLSRHSSPHQpSEDEEE) as substrate.2. Immobilized His-mGSK3B at 10 μg/ml (100 μl/well) can bind biotinylated human HG3C-CTNNB1, EC50 of biotinylated human HG3C-CTNNB1 is 0.15-0.35 μg/ml.

Application Details

Restrictions: For Research Use only

Handling

Format: Frozen, Liquid

Buffer: Supplied as sterile 20 mM Tris, 500 mM NaCl, 25 % glycerol, 0.2 mM DTT, pH 7.4

Storage: -20 °C

Storage Comment: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Target Details

Target: GSK3 beta (GSK3b) (Glycogen Synthase Kinase 3 beta (GSK3b))

Alternative Name: GSK3B (GSK3b Products)

Synonyms: GSK3 Protein, GSK-3 Protein, C86142 Protein, GSK-3beta Protein, 7330414F15Rik Protein, 8430431H08Rik Protein, GSK3beta Protein, fk80d11 Protein, GSK-3[b] Protein, wu:fb68h05 Protein, wu:fk80d11 Protein, gsk-3 Protein, gsk3 Protein, gsk3-beta Protein, gsk3beta Protein, xgsk-3 Protein, glycogen synthase kinase 3 beta Protein, glycogen synthase kinase 3 beta L homeolog Protein, GSK3B Protein, Gsk3b Protein, gsk3b Protein, gsk3b.L Protein

Background:

Background: GSK3B is a serine-threonine kinase, belonging to the glycogen synthase kinase subfamily. It Contains 1 protein kinase domain, and is expressed in testis, thymus, prostate and ovary and weakly expressed in lung, brain and kidney. GSK3B is involved in energy metabolism, neuronal cell development, and body pattern formation. Polymorphisms in GSK3B gene have been implicated in modifying risk of Parkinson disease, and studies in mice show that overexpression of this gene may be relevant to the pathogenesis of Alzheimer disease. GSK3B participates in the Wnt signaling pathway. It is implicated in the hormonal control of several regulatory proteins including glycogen synthase, MYB and the transcription factor JUN. Phosphorylates JUN at sites proximal to its DNA-binding domain, thereby reducing its affinity for DNA. Phosphorylates MUC1 in breast cancer cells, and decreases the interaction of MUC1 with CTNNB1/beta-catenin. GSK3B also plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. It prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization. GSK3B phosphorylates MACF1 and this phosphorylation inhibits the binding of MACF1 to microtubules which is critical for its role in bulge stem cell migration and skin wound repair. It may be required for early embryo development and neuron differentiation.

Synonym: 7330414F15Rik,8430431H08Rik,C86142,GSK-3,GSK-3beta,GSK3

Molecular Weight: 49 kDa

UniProt: Q9WV60

Pathways: WNT Signaling, Hedgehog Signaling, Fc-epsilon Receptor Signaling Pathway, Cellular Glucan Metabolic Process, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Autophagy, BCR Signaling, Warburg Effect