GPC5 Protein (His tag)

Catalog No. ABIN7318048

Product Details

Target: GPC5 (Glypican 5 (GPC5))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Baculovirus infected Insect Cells

Purification tag / Conjugate: This GPC5 protein is labelled with His tag.

Purpose: Recombinant Human Glypican 5/GPC5 Protein (His Tag)(Active)

Sequence: Met 1-Thr 554

Characteristics: A DNA sequence encoding the human GPC5 (NP_004457.1) (Met 1-Thr 554) was fused with a polyhistidine tag at the C-terminus.

Purity: > 92 % as determined by reducing SDS-PAGE.

Endotoxin Level: < 1.0 EU per μg as determined by the LAL method.

Biological Activity Comment: Measured by its binding ability in a functional ELISA. Immobilized human GPC5 at 5 μg/ml (100 μl/well) can bind human bFGF with a linear ranger of 0.156-2.5 ng/ml.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile 50 mM Tris, 100 mM NaCl, pH 8.0

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: GPC5 (Glypican 5 (GPC5))

Alternative Name: Glypican 5/GPC5 (GPC5 Products)

Synonyms: GPC5 Protein, A230034F01Rik Protein, glypican 5 Protein, glypican-5 Protein, GPC5 Protein, Gpc5 Protein, LOC100719080 Protein

Background:

Background: Glypican-5 (GPC5), is a cell membrane protein which belongs to the glypican family. The glypicans compose a family of glycosylphosphatidylinositol-anchored heparan sulfate proteoglycans that may play a role in the control of cell division and growth regulation. So far, six members (Glypican-1/GPC1, Glypican-2/GPC2, Glypican-3/GPC3, Glypican-4/GPC4, Glypican-5/GPC5, Glypican-6/GPC6) of this family are known in vertebrates. In adult, Glypican-5 is primarily expressed in the brain. It is also detected in fetal brain, lung and liver. Glypican-5 enhances the intracellular signaling of FGF2 and HGF. It alters the cellular distribution of FGF2. The properties of Glypican-5 make it an attractive target for therapeutic intervention in rhabdomyosarcomas and other tumors that amplify and/or overexpress its gene. Glypican-5 is over-expressed in lymphoma cell lines that had shown amplification. It is a likely target for amplification, and that over-expression of GPC5 may contribute to development and/or progression of lymphomas and other tumors.

Synonym: GPC5,bA93M14.1,glypican proteoglycan 5,glypican-5

Molecular Weight: 60.5 kDa

NCBI Accession: NP_004457

Pathways: Glycosaminoglycan Metabolic Process