Insulin Receptor Protein (INSR) (His-GST)

Catalog No. ABIN7317078

This Recombinant Insulin Receptor protein is produced in Baculovirus infected Insect Cells.

Quick Overview for Insulin Receptor Protein (INSR) (His-GST) (ABIN7317078)

Target: Insulin Receptor (INSR)

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Baculovirus infected Insect Cells

Purity: > 92 % as determined by reducing SDS-PAGE.

Product Details

Purification tag / Conjugate: This Insulin Receptor protein is labelled with His-GST.

Purpose: Recombinant Human Insulin Receptor/INSR Protein (His & GST Tag)

Sequence: Gly 989-Ser 1382

Characteristics: A DNA sequence encoding the human INSR isoform long (NP_000199.2) cytoplasmic domain (Gly 989-Ser 1382) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Sterility: 0.2 μm filtered

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: The specific activity was determined to be 45 nmol/min/mg using Poly(Ala, Glu, Lys, Tyr)6:2:5:1 as substrate.

Application Details

Restrictions: For Research Use only

Handling

Buffer: Supplied as sterile 50 mM Tris, 100 mM NaCI, pH 7.5, 25 % glycerol, 1 mM TCEP,0.5 mM GSH

Storage: -20 °C

Storage Comment: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Expiry Date: 6 months

Target Details

Target: Insulin Receptor (INSR)

Alternative Name: Insulin Receptor/INSR

Background: CD220,HHF5,Insulin Receptor,INSR (Insulin receptor), also known as CD220, is a transmembrane receptor that is activated by insulin. INSR belongs to theprotein kinase superfamily, and exists as a tetramer consisting of two alpha subunits and two beta subunits linked by disulfide bonds. The alpha and beta subunits are encoded by a single INSR gene, and the beta subunits pass through the cellular membrane. As the receptor for insulin with tyrosine-protein kinase activity, INSR associates with downstream mediators upon binding to insulin, including IRS1 (insulin receptor substrate 1) and phosphatidylinositol 3'-kinase (PI3K). IRS-1 binding and phosphorylation eventually leads to an increase in the high affinity glucose transporter (Glut4) molecules on the outer membrane of insulin-responsive tissues. INSR isoform long and isoform short are expressed in the peripheral nerve, kidney, liver, striated muscle, fibroblasts and skin, and is found as a hybrid receptor with IGF1R which also binds IGF1 in muscle, heart, kidney, adipose tissue, skeletal muscle, hepatoma, fibrobasts, spleen and placenta. Defects in Insulin Receptor/INSR are the cause of Rabson-Mendenhall syndrome (Mendenhall syndrome), insulin resistance (Ins resistance), leprechaunism (Donohue syndrome), and familial hyperinsulinemic hypoglycemia 5 (HHF5). It may also be associated with noninsulin-dependent diabetes mellitus (NIDDM).

Molecular Weight:

Calculated MW: 72.3 kDa

Observed MW: 70 kDa

Gene ID: 3643

NCBI Accession: NP_000199

UniProt: P06213

Pathways: NF-kappaB Signaling, RTK Signaling, AMPK Signaling, Carbohydrate Homeostasis, Regulation of Cell Size, Regulation of Carbohydrate Metabolic Process, Growth Factor Binding, Negative Regulation of Transporter Activity