IL10RB Protein (His tag,Fc Tag)

Catalog No. ABIN7196366

Product Details

Target: IL10RB (Interleukin 10 Receptor, beta (IL10RB))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This IL10RB protein is labelled with His tag,Fc Tag.

Purpose: Recombinant Human IL10RB/IL10R2 Protein (His & Fc Tag)(Active)

Sequence: Met 1-Ser 220

Characteristics: DNA sequence encoding the extracellular domain (Met 1-Ser 220) of human IL10Rβ (NP_000619.3) was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.

Purity: > 92 % as determined by reducing SDS-PAGE.

Endotoxin Level: < 1.0 EU per μg as determined by the LAL method.

Biological Activity Comment: Measured by its binding ability in a functional ELISA. Immobilized human IL28B at 20 μg/ml (100 μl/well) can bind human IL10RB-Fc with a linear ranger of 3.125-25 μg/ml.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, pH 7.4

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: IL10RB (Interleukin 10 Receptor, beta (IL10RB))

Alternative Name: IL10RB/IL10R2 (IL10RB Products)

Synonyms: IL10R2 Protein, IL10RB Protein, il10r2 Protein, CDW210B Protein, CRF2-4 Protein, CRFB4 Protein, D21S58 Protein, D21S66 Protein, IL-10R2 Protein, 6620401D04Rik Protein, AI528744 Protein, Crfb4 Protein, D16H21S58 Protein, D21S58h Protein, Il10r2 Protein, RGD1560373 Protein, interleukin 10 receptor subunit beta Protein, interleukin 10 receptor subunit beta S homeolog Protein, interleukin-10 receptor subunit beta Protein, interleukin 10 receptor, beta Protein, IL10RB Protein, il10rb.S Protein, LOC473966 Protein, LOC478404 Protein, il10rb Protein, Il10rb Protein

Background:

Background: Interleukin 10 receptor, beta subunit (IL10RB/IL-10RB) also known as Cytokine receptor family 2 member 4, Interleukin-10 receptor subunit 2, and cytokine receptor family II, member 4, is a subunit for the interleukin-10 receptor. IL10RB/IL-10RB belongs to the cytokine receptor family. It is an accessory chain essential for the active interleukin 10 receptor complex. Coexpression of this and IL10RA proteins has been shown to be required for IL10-induced signal transduction. Defects in IL10RB/IL-10RB are the cause of inflammatory bowel disease type 25 (IBD25). It is a chronic, relapsing inflammation of the gastrointestinal tract with a complex etiology. It is subdivided into Crohn disease and ulcerative colitis phenotypes. Crohn disease may affect any part of the gastrointestinal tract from the mouth to the anus, but most frequently it involves the terminal ileum and colon. Bowel inflammation is transmural and discontinuous, it may contain granulomas or be associated with intestinal or perianal fistulas. In contrast, in ulcerative colitis, the inflammation is continuous and limited to rectal and colonic mucosal layers, fistulas and granulomas are not observed. Both diseases include extraintestinal inflammation of the skin, eyes, or joints.

Synonym: CDW210B,CRF2-4,CRFB4,D21S58,D21S66,IL-10R2,Interleukin-10 receptor subunit beta(IL10RB),Cytokine receptor class-II member 4,Cytokine receptor family 2 member 4,Interleukin-10 receptor subunit 2

Molecular Weight: 51.7 kDa

NCBI Accession: NP_000619