IL12 Protein (AA 23-215, AA 23-335)

Catalog No. ABIN7320573

This Recombinant IL12 protein is produced in HEK-293 Cells.

Quick Overview for IL12 Protein (AA 23-215, AA 23-335) (ABIN7320573)

Target: IL12 (Interleukin 12 (IL12))

Protein Type: Recombinant

Origin: Mouse

Source: HEK-293 Cells

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 23-215, AA 23-335

Purpose: Recombinant Mouse Interleukin-12/IL-12 Protein

Sequence: Met23-Ser335&Arg23-Ala215

Characteristics: Recombinant Mouse Interleukin-12 is produced by our Mammalian expression system and the target gene encoding Met23-Ser335&Arg23-Ala215 is expressed.

Sterility: 0.2 μm filtered

Endotoxin Level: < 0.01 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Not validated for activity

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: IL12 (Interleukin 12 (IL12))

Alternative Name: Interleukin-12/IL-12

Background: CLMF p35,CLMF p40,Cytotoxic lymphocyte maturation factor 35 kDa subunit,Cytotoxic lymphocyte maturation factor 40 kDa subunit,IL-12,IL-12 subunit p35,IL-12 subunit p40,IL-12A,IL-12B,Interleukin 12,Interleukin-12 subunit alpha,Interleukin-12 subunit beta,Mouse IL-12 is a heterodimeric pleiotropic cytokine made up of a 40 kDa (p40) subunit and a 35 kDa (p35) subunit. Human and mouse IL-12 share 70 % and 60 % amino acid sequence identity in their p40 and p35 subunits, respectively. While mouse IL-12 is active on both human and mouse cells, human IL-12 is not active on murine cells. It is involved in the differentiation of naive T cells into Th1 cells. It is known as a T cell-stimulating factor, which can stimulate the growth and function of T cells. It stimulates the production of interferon-gamma (IFN-γ) and tumor necrosis factor-alpha (TNF-α) from T cells and natural killer (NK) cells, and reduces IL-4 mediated suppression of IFN-γ. T cells that produce IL-12 have a coreceptor, CD30, which is associated with IL-12 activity. IL-12 plays an important role in the activities of natural killer cells and T lymphocytes.IL-12 mediates enhancement of the cytotoxic activity of NK cells and CD8+ cytotoxic T lymphocytes.

Molecular Weight:

Calculated MW: 35.8&21.7 kDa

Observed MW: 40-55&20-28 kDa

UniProt: P43432, P43431

Pathways: JAK-STAT Signaling, TLR Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation, Cancer Immune Checkpoints, Inflammasome