IL-6 Protein (AA 25-211)

Catalog No. ABIN7321248

This Recombinant IL-6 protein is produced in Escherichia coli (E. coli).

Quick Overview for IL-6 Protein (AA 25-211) (ABIN7321248)

Target: IL-6 (IL6) (Interleukin 6 (IL6))

Protein Type: Recombinant

Biological Activity: Active

Origin: Rat

Source: Escherichia coli (E. coli)

Application: Cell Culture (CC)

Purity: > 90 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 25-211

Purpose: Recombinant Rat IL-6/Interleukin-6 Protein

Sequence: Phe25-Thr211

Characteristics: A DNA sequence encoding the rat IL6 (Phe25-Thr211) was expressed and purified with an initial Met.

Sterility: 0.2 μm filtered

Biological Activity Comment: Measured in a cell proliferation assay using T1165 mouse plasmacytoma cells. The ED₅₀ for this effect is typically 1-6 ng/ml.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from sterile 40 % acetonitrile, 0.1 % TFA
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: IL-6 (IL6) (Interleukin 6 (IL6))

Alternative Name: IL-6/Interleukin-6

Background: IL6,Interleukin-6 (IL-6) is a multifunctional α-helical cytokine that regulates cell growth and differentiation of various tissues, which is known particularly for its role in the immune response and acute phase reactions. IL-6 protein is secreted by a variety of cell types including T cells and macrophages as phosphorylated and variably glycosylated molecule. It exerts actions through the its heterodimeric receptor composed of IL-6R that lacks the tyrosine/kinase domain and binds IL-6 with low affinity, and ubiquitously expressed glycoprotein 130 (gp130) that binds the IL-6. IL-6R complex with high affinity and thus transduces signals. IL-6 is also involved in hematopoiesis, bone metabolism, and cancer progression, and has been defined an essential role in directing transition from innate to acquired immunity.

Molecular Weight:

Calculated MW: 21.9 kDa

Observed MW: 22 kDa

NCBI Accession: NP_036721

UniProt: A6KJN3

Pathways: TLR Signaling, Hormone Transport, Negative Regulation of Hormone Secretion, Myometrial Relaxation and Contraction, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Regulation of Carbohydrate Metabolic Process, Autophagy, Cell RedoxHomeostasis, Cancer Immune Checkpoints, Inflammasome