Kallikrein 13 Protein (His tag)

Catalog No. ABIN7196676

Product Details

Target: Kallikrein 13 (KLK13) (Kallikrein-Related Peptidase 13 (KLK13))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This Kallikrein 13 protein is labelled with His tag.

Purpose: Recombinant Human Kallikrein 13/KLK13 Protein (His Tag)(Active)

Sequence: Met 1-Ile 262

Characteristics: A DNA sequence encoding the pro-form of human KLK13 (NP_056411.1) (Met 1-Ile 262) was expressed with a C-terminal polyhistidine tag.

Purity: > 95 % as determined by reducing SDS-PAGE.

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Measured by its ability to cleave the fluorogenic peptide substrate Boc-VPR-AMC (R&D Systems, Catalog # ES011). The specific activity is >200 pmoles/min/μg.(Activation description: The proenzyme needs to be activated by Lysyl-Endopeptidase for an activated form)

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, pH 7.4

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: Kallikrein 13 (KLK13) (Kallikrein-Related Peptidase 13 (KLK13))

Alternative Name: Kallikrein 13/KLK13 (KLK13 Products)

Synonyms: KLK-L4 Protein, KLKL4 Protein, Egfbp-2 Protein, mGk-13 Protein, kallikrein related peptidase 13 Protein, kallikrein related-peptidase 13 Protein, KLK13 Protein, Klk13 Protein

Background:

Background: Tissue kallikrein 13 (hK13), also known as KLK-L4 (kallikrein-like gene 4), is a member of the human tissue kallikrein family of serine proteases having diverse physiological functions in many tissues. The KLK13 gene resides on chromosome 19q13.3-4 along with other 14 members in a gene cluster and shares a high degree of homology. KLK13 is a trypsin-like, secreted serine protease expressed specifically in the testicular tissue including prostate, salivary gland, breast, and testis. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and may play a role in metastasis. KLK13 may be involved in the pathogenesis and/or progression of breast and ovary cancers, and is regarded as a novel cancer biomarker. In addition, KLK13 interacts and forms complexes with several serum protease inhibitors, such as alpha2-macroglobulin, and its expression is regulated by steroid hormones.

Synonym: Kallikrein-13, Kallikrein-Like Protein 4, KLK-L4, KLK13, KLKL4

Molecular Weight: 28.4 kDa

NCBI Accession: NP_056411

Pathways: Complement System