Low Affinity Immunoglobulin gamma Fc Region Receptor IV (FCGR4) (Active) protein (Biotin,His-Avi Tag)

Catalog No. ABIN7320420

Product Details

Target: Low Affinity Immunoglobulin gamma Fc Region Receptor IV (FCGR4)

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: HEK-293 Cells

Purification tag / Conjugate: Biotin,His-Avi Tag

Purpose: Recombinant Mouse CD16-2/FCGR4 Protein (His&AVI Tag), Biotinylated(Active)

Sequence: Met 1-Gln 203

Characteristics: The amino acid sequence corronding to (Met 1-Gln 203) of mouse FCGR4 (NP_653142.2) was fused with a c-terminal polyhistidine tagged AVI tag at the C-terminus. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.

Purity: > 95 % as determined by SDS-PAGE

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: 1. Measured by its ability to bind human IgG1 (Fc) in a functional ELISA.2. Labeling ratio of biotin to protein: 0.6

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, pH 7.4

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: Low Affinity Immunoglobulin gamma Fc Region Receptor IV (FCGR4)

Alternative Name: CD16-2/FCGR4

Background:

Background: Fcgr4, also known as CD16-2, is one of the receptors for Fc region of IgG which involve in immune responses. Fcgr4 mainly functions in cellular response to lipopolysaccharide, NK T cell proliferation, regulation of sensory perception of pain, wound healing etc. Three groups are included for Fc γ receptors (FcR), and they are Fc γ RI (CD64), Fc γ RII (CD32), and Fc γ RIII (CD16). Among these, CD64 possess high affinity even for monomeric IgG, while CD32 and CD16 display a relative lower affinity for IgG. Genes encodes these receptors are diverse differing by species and cell types. The aggregation of FcR having immunoreceptor tyrosine-based activation motifs (ITAMs) activates sequentially src family tyrosine kinases and syk family tyrosine kinases that connect transduced signals to common activation pathways shared with other receptors. FcR with ITAMs elicit cell activation, endocytosis, and phagocytosis. Fcgr4 belongs to Fc γ RIII (CD16) group.

Synonym: 4833442P21Rik Protein, Mouse,CD16-2 Protein, Mouse,FcgammaRIV Protein, Mouse,Fcgr3a Protein, Mouse,FcgRIV Protein, Mouse,Fcrl3 Protein, Mouse

Molecular Weight: 24.2 kDa

NCBI Accession: NP_653142