MARK3 Protein (GST tag,His tag)

Catalog No. ABIN7317055

Product Details

Target: MARK3 (MAP/microtubule Affinity-Regulating Kinase 3 (MARK3))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Baculovirus infected Insect Cells

Purification tag / Conjugate: This MARK3 protein is labelled with GST tag,His tag.

Purpose: Recombinant Human MARK3/CTAK1/EMK-2 Protein (His & GST Tag)(Active)

Sequence: Met 1-Leu 729

Characteristics: A DNA sequence encoding full length of human MARK3 (AAH24773.1) (Met 1-Leu 729) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Purity: > 88 % as determined by reducing SDS-PAGE.

Endotoxin Level: < 1.0 EU per μg as determined by the LAL method.

Biological Activity Comment: The specific activity was determined to be 7 nmol/min/mg using synthetic CHKtide peptide (KKKVSRSGLYRSPSMPENLNRPR) as substrate.

Application Details

Restrictions: For Research Use only

Handling

Format: Frozen, Liquid

Buffer: Supplied as sterile 20 mM Tris, 500 mM NaCl, 0.25 mM DTT, 0.1 mM PMSF, 0.1 mM EDTA, pH 7.4, 10 % gly

Storage: -20 °C

Storage Comment: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Target Details

Target: MARK3 (MAP/microtubule Affinity-Regulating Kinase 3 (MARK3))

Alternative Name: MARK3/CTAK1/EMK-2 (MARK3 Products)

Synonyms: MARK3 Protein, par1 Protein, par-1 Protein, Par-1A Protein, CTAK1 Protein, KP78 Protein, PAR1A Protein, Par-1a Protein, 1600015G02Rik Protein, A430080F22Rik Protein, C-TAK1 Protein, ETK-1 Protein, ETK1 Protein, Emk2 Protein, MPK10 Protein, fi39g08 Protein, wu:fi39g08 Protein, zgc:152848 Protein, zgc:55693 Protein, c-tak1 Protein, ctak1 Protein, emk-2 Protein, microtubule affinity regulating kinase 3 Protein, MAP/microtubule affinity-regulating kinase 3b Protein, MAP/microtubule affinity regulating kinase 3 Protein, MAP/microtubule affinity-regulating kinase 3a Protein, microtubule affinity regulating kinase 3 L homeolog Protein, MARK3 Protein, mark3b Protein, mark3 Protein, Mark3 Protein, mark3a Protein, mark3.L Protein

Background:

Background: MAP / microtubule affinity-regulating kinase 3, also known as C-TAK1, cTAK1, Cdc25C-associated protein kinase 1, ELKL motif kinase 2, Protein kinase STK10, Ser/Thr protein kinase PAR-1, Serine/threonine-protein kinase p78, MARK3, CTAK1 and EMK2, is a ubiquitous expressed protein which belongs to the protein kinase superfamily, CAMK Ser / Thr protein kinase family and MARK subfamily. MARK3 contains one KA1 (kinase-associated) domain, one protein kinase domain and one UBA domain. The Par-1 / MARK protein kinases play a pivotal role in establishing cellular polarity. This family of kinases contains a unique domain architecture, in which a ubiquitin-associated (UBA) domain is located C-terminal to the kinase domain. MARKs / PAR-1 are common regulators of cell polarity that are conserved from nematode to human. All of these kinases have a highly conserved C-terminal domain, which is termed the kinase-associated domain 1 (KA1).

Synonym: CTAK1;KP78;Par-1a;PAR1A

Molecular Weight: 109.3 kDa

Pathways: SARS-CoV-2 Protein Interactome