TRKA Protein (His tag)

Catalog No. ABIN7321090

Product Details

Target: TRKA (NTRK1) (Neurotrophic Tyrosine Kinase, Receptor, Type 1 (NTRK1))

Protein Type: Recombinant

Biological Activity: Active

Origin: Rat

Source: HEK-293 Cells

Purification tag / Conjugate: This TRKA protein is labelled with His tag.

Purpose: Recombinant Rat TrkA/NTRK1 Protein (His Tag)(Active)

Sequence: Met1-Pro418

Characteristics: A DNA sequence encoding the rat NTRK1 (Met1-Pro418) was expressed with a polyhistidine tag at the C-terminus.

Purity: > 95 % as determined by SDS-PAGE

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method

Biological Activity Comment: Measured by its ability to inhibit NGF-induced proliferation of TF-1 human erythroleukemic cells.The ED50 for this effect is typically 0.2-0.8 μg/mL in the presence of 10 ng/mL of recombinant mouse NGF.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, pH 7.4

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: TRKA (NTRK1) (Neurotrophic Tyrosine Kinase, Receptor, Type 1 (NTRK1))

Alternative Name: TrkA/NTRK1 (NTRK1 Products)

Synonyms: MTC Protein, TRK Protein, TRK1 Protein, TRKA Protein, Trk-A Protein, p140-TrkA Protein, CDHF12 Protein, CDHR16 Protein, HSCR1 Protein, MEN2A Protein, MEN2B Protein, MTC1 Protein, PTC Protein, RET-ELE1 Protein, RET51 Protein, Trk Protein, C80751 Protein, Tkr Protein, TrkA Protein, trk Protein, trka Protein, CTRKA Protein, trkA Protein, neurotrophic receptor tyrosine kinase 1 Protein, ret proto-oncogene Protein, neurotrophic tyrosine kinase, receptor, type 1 Protein, NTRK1 Protein, RET Protein, Ntrk1 Protein, ntrk1 Protein

Background:

Background: TRKA is a member of the neurotrophic tyrosine kinase receptor (NTKR) family. It is a membrane-bound receptor that, upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. Isoform TrkA-III promotes angiogenesis and has oncogenic activity when overexpressed. Isoform TrkA-I is found in most non-neuronal tissues. Isoform TrkA-II is primarily expressed in neuronal cells. TrkA-III is specifically expressed by pluripotent neural stem and neural crest progenitors. The presence of NTRK1 leads to cell differentiation and may play a role in specifying sensory neuron subtypes. Mutations in TRKA gene have been associated with congenital insensitivity to pain, anhidrosis, self-mutilating behavior, mental retardation and cancer. It was originally identified as an oncogene as it is commonly mutated in cancers, particularly colon and thyroid carcinomas. TRKA is required for high-affinity binding to nerve growth factor (NGF), neurotrophin-3 and neurotrophin-4/5 but not brain-derived neurotrophic factor (BDNF). Known substrates for the Trk receptors are SHC1, PI 3-kinase, and PLC-gamma-1. NTRK1 has a crucial role in the development and function of the nociceptive reception system as well as establishment of thermal regulation via sweating. It also activates ERK1 by either SHC1- or PLC-gamma-1-dependent signaling pathway. Defects in NTRK1 are a cause of congenital insensitivity to pain with anhidrosis and thyroid papillary carcinoma.Immune Checkpoint   Immunotherapy   Cancer Immunotherapy   Targeted Therapy

Synonym: NTRK1;Trk;Trka

Molecular Weight: 43.7 kDa

NCBI Accession: NP_067600

Pathways: RTK Signaling, Neurotrophin Signaling Pathway, cAMP Metabolic Process