OPCML Protein (AA 1-541) (His tag)

Catalog No. ABIN7320094

Product Details

Target: OPCML (Opioid Binding Protein/cell Adhesion Molecule-Like (OPCML))

Protein Type: Recombinant

Biological Activity: Active

Protein Characteristics: AA 1-541

Origin: Mouse

Source: HEK-293 Cells

Purification tag / Conjugate: This OPCML protein is labelled with His tag.

Purpose: Recombinant Mouse OBCAM/OPCML Protein (aa 1-541, His Tag)(Active)

Sequence: Met 1-Ala 541

Characteristics: A DNA sequence encoding the extracellular domain of mouse BCAM (Q9R069) (Met 1-Ala 541) was expressed, with a C-terminal polyhistidine tag.

Purity: > 98 % as determined by SDS-PAGE

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Measured by the ability of the immobilized protein to support the adhesion of HOS human osteogenic sarcoma cells.When cells are added to coated plates(5 μg/mL, 100μL/well), approximately 30-70% will adhere after 1 hour at 37°C.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, pH 7.4

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: OPCML (Opioid Binding Protein/cell Adhesion Molecule-Like (OPCML))

Alternative Name: OBCAM/OPCML (OPCML Products)

Synonyms: IGLON1 Protein, OBCAM Protein, OPCM Protein, 2900075O15Rik Protein, 3732419F12 Protein, AI844366 Protein, B930023M13Rik Protein, C230027C17 Protein, Gm181 Protein, Obcam Protein, opioid binding protein/cell adhesion molecule like Protein, opioid binding protein/cell adhesion molecule-like Protein, OPCML Protein, Opcml Protein

Background:

Background: The Lutheran (Lu) blood group and basal cell adhesion molecule (BCAM) antigens are both carried by 2 glycoprotein isoforms of the immunoglobulin superfamily representing receptors for the laminin alpha(5) chain. It is a transmembrane receptor with five immunoglobulin-like domains in its extracellular region, and is therefore classified as a member of the immunoglobulin (Ig) gene family. In addition to red blood cells, Lu/BCAM proteins are expressed in endothelial cells of vascular capillaries and in epithelial cells of several tissues. BCAM/LU has a wide tissue distribution with a predominant expression in the basal layer of the epithelium and the endothelium of blood vessel walls. As designated as CD239 recently, BCAM and LU share a significant sequence similarity with the CD146 (MUC18) and CD166, and themselves are adhesion molecules that bind laminin with high affinity. Laminins are found in all basement membranes and are involved in cell differentiation, adhesion, migration, and proliferation. BCAM is upregulated following malignant transformation of some cell types in vivo and in vitro, thus being a candidate molecule involved in tumor progression. In addition, BCAM interacts with integrin in sickle red cells, and thus may potentially play a role in vaso-occlusive episodes.

Synonym: 1200005K12Rik,B-CAM,Gplu,Lu

Molecular Weight: 58.3 kDa

UniProt: Q9R069