OTUB1 Protein (His tag)
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- Target See all OTUB1 Proteins
- OTUB1 (OTU Domain, Ubiquitin Aldehyde Binding 1 (OTUB1))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This OTUB1 protein is labelled with His tag.
- Purpose
- Recombinant Human OTUB1/OTB1 Protein (His Tag)
- Sequence
- Met 1-Lys 271
- Characteristics
- A DNA sequence encoding the human OTUB1 (Q96FW1-1) (Met 1-Lys 271) was expressed, with a polyhistide tag at the N-terminus.
- Purity
- > 97 % as determined by reducing SDS-PAGE.
- Top Product
- Discover our top product OTUB1 Protein
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Please refer to the printed manual for detailed information.
- Buffer
- Lyophilized from sterile PBS, 20 % glycerol, pH 7.4
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- Target
- OTUB1 (OTU Domain, Ubiquitin Aldehyde Binding 1 (OTUB1))
- Alternative Name
- OTUB1/OTB1 (OTUB1 Products)
- Synonyms
- OTB1 Protein, OTU1 Protein, AI850305 Protein, fa19h07 Protein, otub1 Protein, wu:fa19h07 Protein, zgc:92839 Protein, MGC131231 Protein, fb17f04 Protein, otub1l Protein, wu:fb17f04 Protein, zgc:92685 Protein, OTU deubiquitinase, ubiquitin aldehyde binding 1 Protein, OTU domain, ubiquitin aldehyde binding 1 Protein, OTU deubiquitinase, ubiquitin aldehyde binding 1b Protein, OTU deubiquitinase, ubiquitin aldehyde binding 1 L homeolog Protein, similar to HSPC263 Protein, OTU deubiquitinase, ubiquitin aldehyde binding 1a Protein, OTUB1 Protein, Otub1 Protein, otub1b Protein, otub1.L Protein, otub1 Protein, RGD1565010 Protein, otub1a Protein
- Background
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Background: Ubiquitin thioesterase OTUB1, also known as Deubiquitinating enzyme OTUB1, OTU domain-containing ubiquitin aldehyde-binding protein 1, Otubain-1, Ubiquitin-specific-processing protease OTUB1, OTUB1 and OTB1, is a cytoplasm protein which belongs to the peptidase C65 family. OTUB1 is a hydrolase that can remove conjugated ubiquitin from proteins and plays an important regulatory role at the level of protein turnover by preventing degradation. OTUB1 is a regulator of T-cell anergy, a phenomenon that occurs when T-cells are rendered unresponsive to antigen rechallenge and no longer respond to their cognate antigen. OTUB1 acts via its interaction with RNF128 / GRAIL, a crucial inductor of CD4 T-cell anergy. Isoform 1 of OTUB1 destabilizes RNF128, leading to prevent anergy. In contrast, isoform 2 of OTUB1 stabilizes RNF128 and promotes anergy. OTUB1 regulates RNF128-mediated ubiquitination, but does not deubiquitinate polyubiquitinated RNF128. Deubiquitinates estrogen receptor alpha (ESR1). OTUB1 mediates deubiquitination of 'Lys-48'-linked polyubiquitin chains, but not 'Lys-63'-linked polyubiquitin chains. OTUB1 is also capable of removing NEDD8 from NEDD8 conjugates, but with a much lower preference compared to 'Lys-48'-linked ubiquitin.
Synonym: HSPC263;OTB1;OTU1
- Molecular Weight
- 32.8 kDa
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