PDE3A Protein (GST tag,His tag)
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- Target See all PDE3A Proteins
- PDE3A (phosphodiesterase 3A, cGMP-Inhibited (PDE3A))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- Baculovirus infected Insect Cells
- Purification tag / Conjugate
- This PDE3A protein is labelled with GST tag,His tag.
- Purpose
- Recombinant Human PDE3A Protein (His & GST Tag)
- Sequence
- Lys669-Gln1141
- Characteristics
- A DNA sequence encoding the human PDE3A (Q14432) (Lys669-Gln1141) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
- Purity
- > 90 % as determined by reducing SDS-PAGE.
- Endotoxin Level
- < 1.0 EU per μg as determined by the LAL method.
- Top Product
- Discover our top product PDE3A Protein
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phosphodiesterase 3A, cGMP-Inhibited (PDE3A) (AA 533-640) protein (GST tag)
PDE3A Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
phosphodiesterase 3A, cGMP-Inhibited (PDE3A) (AA 1-1141) protein (Strep Tag)Crystallography grade PDE3A Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
phosphodiesterase 3A, cGMP-Inhibited (PDE3A) (AA 1-1141) protein (Strep Tag)Crystallography grade PDE3A Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Please refer to the printed manual for detailed information.
- Buffer
- Lyophilized from sterile 20 mM Tris, 500 mM NaCl, pH 7.4, 10 % glycerol, 3 mM DTT
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- Target
- PDE3A (phosphodiesterase 3A, cGMP-Inhibited (PDE3A))
- Alternative Name
- PDE3A (PDE3A Products)
- Synonyms
- PDE3A Protein, Pde3a Protein, si:dkey-48h7.2 Protein, A930022O17Rik Protein, C87899 Protein, RNPDE3A Protein, CGI-PDE Protein, CGI-PDE A Protein, CGI-PDE-A Protein, phosphodiesterase 3A Protein, phosphodiesterase 3A, cGMP-inhibited Protein, cGMP-inhibited 3',5'-cyclic phosphodiesterase A Protein, phosphodiesterase 3A, cGMP inhibited Protein, PDE3A Protein, Pde3a Protein, LOC100540515 Protein, pde3a Protein
- Background
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Background: PDE3A belongs to the phosphodiesterase family. Phosphodiesterases (PDEs) are a family of related phosphohydrolyases that selectively catalyze the hydrolysis of 3' cyclic phosphate bonds in adenosine and/or guanine 3',5' cyclic monophosphate (cAMP and/or cGMP). They regulate the cellular levels, localization and duration of action of these second messengers by controlling the rate of their degradation. PDEs are expressed ubiquitously, with each subtype having a specific tissue distribution. These enzymes are involved in many signal transduction pathways and their functions include vascular smooth muscle proliferation and contraction, cardiac contractility, platelet aggregation, hormone secretion, immune cell activation, and they are involved in learning and memory. PDE3A has high affinity for both cAMP and cGMP and shows competitive inhibition of the cAMP hydrolytic activity by cGMP. It plays a critical role in regulating intracellular levels of cAMP and cGMP.
Synonym: CGI-PDE,CGI-PDE-A,CGI-PDEA
- Molecular Weight
- 81.7 kDa
- UniProt
- Q14432
- Pathways
- cAMP Metabolic Process
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