PRSS27 Protein (His tag)
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- Target See all PRSS27 Proteins
- PRSS27 (Protease, Serine 27 (PRSS27))
- Protein Type
- Recombinant
- Biological Activity
- Active
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This PRSS27 protein is labelled with His tag.
- Purpose
- Recombinant Human Pancreasin/Marapsin/PRSS27 Protein (His Tag)(Active)
- Sequence
- Met 1-Lys 290
- Characteristics
- A DNA sequence encoding the human PRSS27 (Q9BQR3) extracellular domain (Met 1-Lys 290) was expressed, fused with a polyhistidine tag at the C-terminus.
- Purity
- > 95 % as determined by reducing SDS-PAGE.
- Endotoxin Level
- < 1.0 EU per μg as determined by the LAL method.
- Biological Activity Comment
- Measured by its ability to cleave a colorimetric peptide substrate, NcarbobenzyloxyGlyArgThioBenzyl ester (ZGR-SBzl), in the presence of 5,5'Dithiobis (2-nitrobenzoic acid) (DTNB).The specific activity is > 2000 pmols/min/μg.
- Top Product
- Discover our top product PRSS27 Protein
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Protease, Serine 27 (PRSS27) (AA 94-203) protein (GST tag)
PRSS27 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Please refer to the printed manual for detailed information.
- Buffer
- Lyophilized from sterile PBS, pH 7.4
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- Target
- PRSS27 (Protease, Serine 27 (PRSS27))
- Alternative Name
- Pancreasin/Marapsin/PRSS27 (PRSS27 Products)
- Synonyms
- PRSS27 Protein, mpn Protein, caph2 Protein, tmprss9 Protein, marapsin Protein, pancreasin Protein, MGC97879 Protein, CAPH2 Protein, MPN Protein, Mpn Protein, protease, serine 27 Protein, epidermis specific serine protease S homeolog Protein, PRSS27 Protein, prss27 Protein, xepsin.S Protein, Prss27 Protein
- Background
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Background: The name "Pancreasin" because it is transcribed strongly in the pancreas. This secreted, tryptic serine protease, also known as Marapsin or PRSS27 (Protease, serine, 27), which is a member of the peptidase S1 family. Pancreasin is inhibited by benzamidine and leupeptin but resists several classic inhibitors of trypsin. Marapsin was constitutively expressed in nonkeratinizing stratified squamous epithelia of human esophagus, tonsil, cervix, larynx, and cornea. In fact, marapsin was the second most strongly up-regulated protease in psoriatic lesions, where expression was localized to the upper region of the hyperplastic epidermis. Similarly, in the hyperproliferative epithelium of regenerating murine skin wounds, marapsin localized to the suprabasal layers, where keratinocytes undergo squamous differentiation. Marapsin's restricted expression, localization, and cytokine-inducible expression suggest a role in the terminal differentiation of keratinocytes in hyperproliferating squamous epithelia.
Synonym: CAPH2;MPN;UNQ1884/PRO4327
- Molecular Weight
- 30.9 kDa
- UniProt
- Q9BQR3
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